Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 22416..22881 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP069981 | ||
| Organism | Escherichia coli strain FDAARGOS_1291 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9ZUY2 |
| Locus tag | I6K21_RS24440 | Protein ID | WP_001230707.1 |
| Coordinates | 22630..22881 (-) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | M7CCF8 |
| Locus tag | I6K21_RS24435 | Protein ID | WP_000801440.1 |
| Coordinates | 22416..22643 (-) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K21_RS24395 (I6K21_24405) | 17814..18029 | - | 216 | WP_000760375.1 | EexN family lipoprotein | - |
| I6K21_RS24400 (I6K21_24410) | 18026..18319 | - | 294 | WP_000134733.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| I6K21_RS24405 (I6K21_24415) | 18396..19625 | - | 1230 | WP_000539543.1 | DNA relaxase/nickase TaxC | - |
| I6K21_RS24410 (I6K21_24420) | 19629..20132 | - | 504 | WP_139455609.1 | relaxosome-coupling protein TaxA | - |
| I6K21_RS24415 (I6K21_24425) | 20493..20624 | - | 132 | WP_001353745.1 | toxin-antitoxin system HicB family antitoxin | - |
| I6K21_RS24420 (I6K21_24430) | 20929..21222 | - | 294 | WP_000954638.1 | hypothetical protein | - |
| I6K21_RS24425 (I6K21_24435) | 21237..21611 | - | 375 | WP_000844303.1 | tail-associated lysozyme | - |
| I6K21_RS24430 (I6K21_24440) | 21663..21962 | - | 300 | WP_000650311.1 | hypothetical protein | - |
| I6K21_RS24435 (I6K21_24445) | 22416..22643 | - | 228 | WP_000801440.1 | hypothetical protein | Antitoxin |
| I6K21_RS24440 (I6K21_24450) | 22630..22881 | - | 252 | WP_001230707.1 | hypothetical protein | Toxin |
| I6K21_RS24445 (I6K21_24455) | 22878..23420 | - | 543 | WP_001083906.1 | hypothetical protein | - |
| I6K21_RS24450 (I6K21_24460) | 23525..24040 | - | 516 | WP_001025395.1 | J domain-containing protein | - |
| I6K21_RS24455 (I6K21_24465) | 24680..25030 | - | 351 | WP_000127482.1 | addiction module antitoxin | - |
| I6K21_RS24460 (I6K21_24470) | 25027..25362 | - | 336 | WP_001147119.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| I6K21_RS24465 (I6K21_24475) | 26617..27483 | + | 867 | WP_001353743.1 | replication initiation protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..34441 | 34441 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9751.13 Da Isoelectric Point: 8.9187
>T192495 WP_001230707.1 NZ_CP069981:c22881-22630 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR
Download Length: 252 bp
>T192495 NZ_CP092431:c8664985-8664629 [Streptomyces deccanensis]
ATGCCCTTCAAGATTGTCGTGGTGGAACCAGCAAGAAGCTGGTTGCACCATCTCCGTCAAACCGACAGGGGCACGCTGAT
CCAGATCACGCAGGCATTCAACGTTCTGCAGATCGAAGGCCCCGCCTTGGGGCGCCCTCTCGTTGACCGGATCACCGGCT
CGGCCGTGCCGAACCTCAAGGAGCTACGGCCCGGCTCATCCGGAGCCAGCGAGGTGAGATTGTTGTTCGTGTTCGATCCC
GAGCGGAACGCCGTGATCCTGGTCGGCGGCGACAAGGCCGGCAACTGGTCCGGGTGGTACCGGACGGCGATCAAGGAAGC
GGAAGAGGCGTACACGGCGTACAGGGAGGAGTCATGA
ATGCCCTTCAAGATTGTCGTGGTGGAACCAGCAAGAAGCTGGTTGCACCATCTCCGTCAAACCGACAGGGGCACGCTGAT
CCAGATCACGCAGGCATTCAACGTTCTGCAGATCGAAGGCCCCGCCTTGGGGCGCCCTCTCGTTGACCGGATCACCGGCT
CGGCCGTGCCGAACCTCAAGGAGCTACGGCCCGGCTCATCCGGAGCCAGCGAGGTGAGATTGTTGTTCGTGTTCGATCCC
GAGCGGAACGCCGTGATCCTGGTCGGCGGCGACAAGGCCGGCAACTGGTCCGGGTGGTACCGGACGGCGATCAAGGAAGC
GGAAGAGGCGTACACGGCGTACAGGGAGGAGTCATGA
Antitoxin
Download Length: 76 a.a. Molecular weight: 8659.98 Da Isoelectric Point: 8.5105
>AT192495 WP_000801440.1 NZ_CP069981:c22643-22416 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK
Download Length: 228 bp
>AT192495 NZ_CP092431:c8664632-8664291 [Streptomyces deccanensis]
ATGAGCGTGAAGAAGCAGAGCACTGCGAACGCCGTCGACTGGGATGACCTCAAGGAAGAGTTCGACTTCTCCCCGGAGGA
GAAGAAGGAGATCGAACAGGGCACCGCCGCGCTCCTGTCCGAAGTGCGCGCTTACCGCCTCGCCGAGGTCCGCAAGCGCC
AGCACATGACGCAGACGGCGGTGGCCAAGATCCTCGGCGTCACCCAGGGGCGCGTCTCAGCCATCGAGAAGGGTGCGCTC
AGCCGCTCCGAGGTCGACACGCTTGCCGCCTACGTCGAAGCCCTCGGCGGAAAGCTCAAGCTCGTGGCCGACTTCGGCGA
CGAATCGCTTGTGCTGGGGTAA
ATGAGCGTGAAGAAGCAGAGCACTGCGAACGCCGTCGACTGGGATGACCTCAAGGAAGAGTTCGACTTCTCCCCGGAGGA
GAAGAAGGAGATCGAACAGGGCACCGCCGCGCTCCTGTCCGAAGTGCGCGCTTACCGCCTCGCCGAGGTCCGCAAGCGCC
AGCACATGACGCAGACGGCGGTGGCCAAGATCCTCGGCGTCACCCAGGGGCGCGTCTCAGCCATCGAGAAGGGTGCGCTC
AGCCGCTCCGAGGTCGACACGCTTGCCGCCTACGTCGAAGCCCTCGGCGGAAAGCTCAAGCTCGTGGCCGACTTCGGCGA
CGAATCGCTTGTGCTGGGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9ZUY2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Y6U8W9 |