Detailed information of TA system TA192486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4527904..4529492
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: I6K21_RS21475
• Protein ID: WP_001125415.1
• Coordinates: 4527904..4529226 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K21_RS21480
• Protein ID: WP_001296726.1
• Coordinates: 4529226..4529492 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS21435 (4522938)	4522938..4523510	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
I6K21_RS21440 (4523661)	4523661..4523813	-	153	WP_001322417.1	amidohydrolase family protein	-
I6K21_RS21445 (4523803)	4523803..4524222	-	420	WP_001322416.1	amidohydrolase family protein	-
I6K21_RS21450 (4524182)	4524182..4524337	-	156	WP_001322415.1	hypothetical protein	-
I6K21_RS21455 (4524440)	4524440..4525387	-	948	WP_000854796.1	aldo/keto reductase	-
I6K21_RS21460 (4525461)	4525461..4526150	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
I6K21_RS21465 (4526159)	4526159..4526467	-	309	WP_001221046.1	hypothetical protein	-
I6K21_RS21470 (4526607)	4526607..4527506	+	900	WP_001057776.1	LysR family transcriptional regulator	-
I6K21_RS21475 (4527904)	4527904..4529226	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K21_RS21480 (4529226)	4529226..4529492	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T192486 WP_001125415.1 NZ_CP069980:c4529226-4527904 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192486 NZ_CP092430:c63188-62775 [Mycolicibacter virginiensis]
GTGAAACCGCTCTCCGGTTCGCCGGCAGAGCAGGTGGTCGTGGACGCCAGTGCCATGGTGGACCTACTTGCTCGCACCCA
GGATCGGTTCACATCGGTACGTGCGCGGCTGGCCGGCACAGTGATGCACGCGCCGGCGCACTTTGATGCCGAGGTGCTAT
CGGCGCTGGGGCGCATGCAACGCGCCGGCGTGCTGACCGTTGCTCAGGTCGGGGCGGCACTGGATCAGCTACGACGGGCA
CCGGTAACTCGGCACGAACTCTCCCCGTTGCTTGCTGGAGCGTGGGCGCGGCGCGAGATTCTGCGCCTCACCGACGCCCT
CTACGTGGAACTTTCGGACACGGCAGATCTGGTGCTGCTCACCACGGACCAGCGGCTGGCGCGTGCTTGGCCTGCGGCCG
AAGCCATCAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT192486 WP_001296726.1 NZ_CP069980:c4529492-4529226 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192486 NZ_CP092430:c63403-63185 [Mycolicibacter virginiensis]
ATGGCTCGGCTGAACGTGTATGTTCCCGACGATTTGGCGGAGCGAGCCAGGGCGCGGGGACTGAACATCTCGGCGCTGAC
TCAGGCCGCGATCAGCGCTGAGTTGGAGAATTCCGCGACCGACAGCTGGCTTGACGGGCTAGAGGCCTGGAGCACAAGCG
CTCGACACGATGACGTGCTGACTGCGCTCGACGCCGCCCGCGATGAGCTCGGAGCGTGA

Structures

Toxin

• AlphaFold DB: U9YVT8