Detailed information of TA system TA192484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4427934..4428572
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: I6K21_RS21020
• Protein ID: WP_000813797.1
• Coordinates: 4427934..4428110 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K21_RS21025
• Protein ID: WP_076611057.1
• Coordinates: 4428156..4428572 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS21000 (4423556)	4423556..4424728	-	1173	WP_001236223.1	BenE family transporter YdcO	-
I6K21_RS21005 (4424820)	4424820..4425356	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
I6K21_RS21010 (4425429)	4425429..4427390	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K21_RS21015 (4427482)	4427482..4427712	-	231	WP_000494244.1	YncJ family protein	-
I6K21_RS21020 (4427934)	4427934..4428110	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K21_RS21025 (4428156)	4428156..4428572	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K21_RS21030 (4428651)	4428651..4430057	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
I6K21_RS21035 (4430302)	4430302..4431447	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
I6K21_RS21040 (4431465)	4431465..4432478	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
I6K21_RS21045 (4432479)	4432479..4433420	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T192484 WP_000813797.1 NZ_CP069980:4427934-4428110 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T192484 NZ_CP092424:207635-208030 [Mycobacterium lentiflavum]
ATGCGAATCTACTTGGACACGTCCGCGTTGGTGAAACTCGTTGTCGTCGAGGCGGAATCGGTAGCGCTGCAAAACTTCCT
CGCTGAGCAACGCCACGACACCCTGTTCTCAGCAGCCCTGGCGCGCACTGAACTGATACGGGCAGTCTCCCCGAACGGGC
CCCAAGCCATTGGTCACGCACGCGACCTCCTGCACGGCTTGGACATCATCACGCTGAGCCGACAGTTACTCGACGACGCA
GGCACGTTGCAGCCACTAACCCTTCGCAGTCTCGACGCCATTCACCTCGTTGCCGCACAGCGTGCCGGCGACACCCTACG
TACGGTGATCACCTATGACGCCCGGATGGTCGCAGCGGCAGCCGAATTGGGGATCCCCACCGCAGCACCAGGCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT192484 WP_076611057.1 NZ_CP069980:4428156-4428572 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192484 NZ_CP092424:207364-207630 [Mycobacterium lentiflavum]
ATGAGGGCGATAGGAGTTCGCGAGCTTCGGCAGAACGCCAGCAAGTACCTCGAGGACGTCGCAGCGGGGGAGTCGATCGA
GATAACTGACCGCGGCCGCCCGGTCGCGCGTCTCGTGCCCGTTACAGGCGATCCATGGCAAGACCTCGTCAACTCCGGCG
AGGTTGTGTTGGCGGCTCGTCCTTTGAAGGCCGACGACCTTGAGCCGCAGAGTTACCCGCACAGTCCCACTGAGGCTCTA
GAACAACTACGGCACGATGAACGCTAG

Structures

Toxin

• AlphaFold DB: A0A829J715