Detailed information of TA system TA192479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4030337..4031121
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K21_RS18895
• Protein ID: WP_000613626.1
• Coordinates: 4030337..4030831 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K21_RS18900
• Protein ID: WP_001110447.1
• Coordinates: 4030828..4031121 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS18875 (4025530)	4025530..4026627	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
I6K21_RS18880 (4026627)	4026627..4027568	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K21_RS18885 (4027634)	4027634..4029277	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
I6K21_RS18890 (4029289)	4029289..4030242	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K21_RS18895 (4030337)	4030337..4030831	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K21_RS18900 (4030828)	4030828..4031121	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K21_RS18905 (4031254)	4031254..4034439	-	3186	WP_000827427.1	ribonuclease E	-
I6K21_RS18910 (4035012)	4035012..4035971	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192479 WP_000613626.1 NZ_CP069980:c4030831-4030337 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192479 NZ_CP092419:c2380936-2380535 [Leptolyngbya boryana IU 594]
ATGCAATATCTGTTGGACACGAACATCTGCATCTATTTGATTAAGCAAAGACCTCCAAAGGTATTGGAGCGATTTTCAAA
TCTTGCTTTGTCAGATATCGGCATATCCTCTATCACTGTGGCGGAACTAGAATATGGCGTTTGCAAAAGCCAGCAACGAG
AGAAAAACCGTAATGCTTTGATGCAATTTTTAATCCCGCTAGAAATCGTTGCGTTTGATCAAGCGGCTGCGACCCTCTAT
GGCGCAATCAGAAGCGATCTAGAAAGTAGAGGACTGGTTATCGGAGCAATGGACATGCTGATTGCTGCCCACGCATTGAG
CCTTAGCGTTACGTTAGTCAGCAACAATGTGCGAGAGTTTTCACGCATTGCCAATCTATCTCTGCAAAACTGGGCAGAAT
GA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192479 WP_001110447.1 NZ_CP069980:c4031121-4030828 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192479 NZ_CP092419:c2381164-2380937 [Leptolyngbya boryana IU 594]
ATGAATATTGCCAAACTTAGCACCGATGGCACCCATCAAATTGTCATACTCCCCGAAGACTGCAAAATGACGGGTACGGA
GGTCTACATTAAAAAAGTTGGAAGTGCGATCGTTCTAATTGCCAAAGATCATCCTTGGCAATCGCTCTTCGATAGCTTAG
AGCAATTCTCTGAAGACTTCATGACCACGAGAGAGCAGCCCCCTCTCGACGTTCGTGAGGCTTTCTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6