Detailed information of TA system TA192478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3742876..3743581
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K21_RS17455
• Protein ID: WP_000539521.1
• Coordinates: 3743195..3743581 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K21_RS17450
• Protein ID: WP_001280945.1
• Coordinates: 3742876..3743205 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS17435 (3738033)	3738033..3738944	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
I6K21_RS17440 (3739122)	3739122..3741470	+	2349	Protein_3427	cyclic diguanylate phosphodiesterase	-
I6K21_RS17445 (3741478)	3741478..3742806	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
I6K21_RS17450 (3742876)	3742876..3743205	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K21_RS17455 (3743195)	3743195..3743581	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K21_RS17460 (3743807)	3743807..3745132	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K21_RS17465 (3745345)	3745345..3745728	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K21_RS17470 (3745839)	3745839..3746954	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
I6K21_RS17475 (3746951)	3746951..3747577	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192478 WP_000539521.1 NZ_CP069980:c3743581-3743195 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192478 NZ_CP092419:2064152-2064493 [Leptolyngbya boryana IU 594]
GTGACAACGATTTGGATCGATGCCCATTTGTCGCCTGTGATCGCAACTTGGATTACAGGCAGATTTGGAATTACCGCGAT
CGCTTTACGGGATATCGGCTTGAGAGATGCCGAAGATCCCGAAATCTTTGAGGCTGCAAAAGCTCAAGGCGTTATATTCA
TGACTAAAGATAGCGATTTCGTGGATCTCGTTGAACGTTTGGAAGCACCGCCACAAATTATTTGGTTAACTTGTGGCAAT
ACATCAAATGCCCGTTTGCAGGAAATTCTAAGCTCGACTTTGCCTGATGCATTAGAGTTATTGAAATCTGGCGAAAAGCT
AGTAGAGATTAGCGGAGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192478 WP_001280945.1 NZ_CP069980:c3743205-3742876 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192478 NZ_CP092419:2063931-2064155 [Leptolyngbya boryana IU 594]
ATGTCAGACTTACTTAAACGAATCACCGTGAATCCTAAGCAATGTGGGGGTCGCCCCTGTATTCGTGGCATGAGAATTCG
AGTGTCCGATGTTTTGGATTTATTTGCAGCAGGGTTAAGTGCTGAACAAATTTTGGAAGAACTGCCTGATCTGGAAAGAG
ATGATTTAAGAGCAGCACTTGCCTACGCTTCACGTAAAGTCAACCACCCTGTTTTAGTCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6