Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3292033..3292651 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K21_RS15310 Protein ID WP_001291435.1
Coordinates 3292033..3292251 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K21_RS15315 Protein ID WP_000344800.1
Coordinates 3292277..3292651 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS15275 (3287322) 3287322..3287894 + 573 WP_000779826.1 YbaY family lipoprotein -
I6K21_RS15280 (3287925) 3287925..3288236 - 312 WP_000409911.1 MGMT family protein -
I6K21_RS15290 (3288615) 3288615..3288968 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K21_RS15295 (3289010) 3289010..3290560 - 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K21_RS15300 (3290724) 3290724..3291194 - 471 WP_000136192.1 YlaC family protein -
I6K21_RS15305 (3291310) 3291310..3291861 - 552 WP_000102559.1 maltose O-acetyltransferase -
I6K21_RS15310 (3292033) 3292033..3292251 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K21_RS15315 (3292277) 3292277..3292651 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K21_RS15320 (3293197) 3293197..3296346 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K21_RS15325 (3296369) 3296369..3297562 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192477 WP_001291435.1 NZ_CP069980:c3292251-3292033 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192477 NZ_CP092419:c1312487-1312224 [Leptolyngbya boryana IU 594]
ATGAGTGGAACAATTCGGACTCGACTTGTCAAATTTGGTAATTCTCAAGGAATTCGGATTCCGAAGCTGTTACTAGAGCA
AAGTGGCATTCAAAGTGAAGTTGAAATCGAAGTCGAAGGCAATCGCTTGATTATTCGTCCTGTATCTCATCCTCGTGCTG
GATGGGAAGCAGCGATCGATGCAGAAATCGCAAAATCTGGGGATGAAGGCTTGCTGGATGATGAAACCTCAACTCAATGG
GACGAAACCGAATGGCAATGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192477 WP_000344800.1 NZ_CP069980:c3292651-3292277 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192477 NZ_CP092419:c1312236-1311910 [Leptolyngbya boryana IU 594]
ATGGCAATGGTAGTTCGTCGTTTTGATGTGTTTCTTGTTCAGCTAGATCCAACTGTTGGATCTGAAATTCAGAAGACCCG
TCCTTGTATAATCGTCTCTCCCGATGAGATGAATCGTTTACTCAAAACGGTTTTGATAGCTCCATTAACTTCTGTAAGAC
GAAACTTCCCATTTCGAGTTGATTGTCAGTTTCAAGGCAAAGCGGGTCAGATTGTCCTAGATCAAATTCGAGCAGTAGAT
AAAACCCGACTTGTTCAATTTCTGGGTCAAATTGGCGAGGAGCAAAGAGCGCTTCTCGATACGATAGCTGACCTCTTTGC
TGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References