Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2413815..2414312 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K21_RS11150 Protein ID WP_001173343.1
Coordinates 2414139..2414312 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K21_RS11145 Protein ID WP_000398619.1
Coordinates 2413815..2414111 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS11125 (2410544) 2410544..2411263 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6K21_RS11130 (2411260) 2411260..2412891 - 1632 WP_001216477.1 sensor histidine kinase -
I6K21_RS11135 (2413072) 2413072..2413302 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K21_RS11140 (2413314) 2413314..2413586 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K21_RS11145 (2413815) 2413815..2414111 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K21_RS11150 (2414139) 2414139..2414312 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K21_RS11155 (2414430) 2414430..2415947 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K21_RS11160 (2416184) 2416184..2417641 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192468 WP_001173343.1 NZ_CP069980:2414139-2414312 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192468 NZ_CP092417:c3265426-3265172 [Endozoicomonas sp. SCSIO W0465]
ATGAAAAAGAAGCATGCCACGACACTGCGCAAGCTCTTTGCCAGGCCAATCAGCGGCAGCATTCACTGGGCGGATATTGA
AAGCTTGTTTATTGCCCTTGGTGCCCGGATCGAAGAACGTGAAGGCAGCCGGGTCAAGGTGGTGCTGTTCGGTGAGGTGC
GGATCTTTCACCGCCCACACCCGTCACCGGATACCGACAAGGGCGCGGTGGCCAGTATCCGCCGGTGGCTGGAAAACAAT
GGAGTGACGCCATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT192468 WP_000398619.1 NZ_CP069980:2413815-2414111 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192468 NZ_CP092417:c3265175-3264843 [Endozoicomonas sp. SCSIO W0465]
ATGATTAACCAGATGACCATTAACGGCTATATCGCCGTCATTACTTACGATCCGGAGATTGACCTGTTCCGGGGTGAATT
CGTCGGCCTGAACGGCGGCGCTGATTTTTACGCGGCGGATGTCAAGAGCCTGCACCAGGAAGGCAAGGTCTCCCTGAAGG
TGTTTCTGGATGCCTGCAAGGAGGAAGGCATTGAACCGCGCAAGGCGTTCTCCGGCAAGCTGAACCTGCGCATTGACCCG
GATCTGCACGAATCCGCCAGTGTCGCTGCCGCCGCCAGTAACCGCAGCCTGAACCAGTTGATTAATGACGCGTTGCGTCA
GTCGCTGAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References