Detailed information of TA system TA192467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1789188..1789800
• Replicon: chromosome
• Accession: NZ_CP069980
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: I6K21_RS08360
• Protein ID: WP_000833473.1
• Coordinates: 1789615..1789800 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: I6K21_RS08355
• Protein ID: WP_000499744.1
• Coordinates: 1789188..1789598 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS08340 (1784700)	1784700..1785515	-	816	WP_000891825.1	AraC family transcriptional regulator	-
I6K21_RS08345 (1785741)	1785741..1787141	+	1401	WP_000204802.1	MFS transporter	-
I6K21_RS08350 (1787152)	1787152..1789116	+	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
I6K21_RS08355 (1789188)	1789188..1789598	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
I6K21_RS08360 (1789615)	1789615..1789800	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
I6K21_RS08365 (1790078)	1790078..1791616	-	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
I6K21_RS08370 (1791817)	1791817..1792968	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T192467 WP_000833473.1 NZ_CP069980:c1789800-1789615 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T192467 NZ_CP092417:c3245135-3244905 [Endozoicomonas sp. SCSIO W0465]
TTGGAAAAACTGAAGGACAACCCGAAAACCTTTCCCTGGAAAGATGTGGTCAGGCTTCTCACGGCACTGGGTTACCAGAA
GGAAGAGATGTCAGGTTCGCGGGTACGCTTTTATCATAAATCGAATAAACATATGCTTCGCTTTCACCGGCCTCACCCGG
AAAACCATCTCAAAGGAGGCGCTTTGAAAGACCTGATTGAAGCATTGAACCAGCAGGATTATTTTTCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT192467 WP_000499744.1 NZ_CP069980:c1789598-1789188 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT192467 NZ_CP092417:c3244908-3244537 [Endozoicomonas sp. SCSIO W0465]
ATGACCCAGTATTTTAACTACAAAGGGTATCAGGGCACCGTTGAACCGCAGATTGAAGACGGCACGCTGTATGGCAAGCT
GGCGTTTATCGGTGACCTGGTGACCTATGAGGCGGACACAATGATAACCCTGGAGCAGGAATTTCGGCGCTCAGTGGACG
ATTACCTGGCGGATTGCGCTGCACTGGGCAAAGAACCCGACAAGCCATTCAAGGGAGTACTGAATGTTCGCCTGGGTGCA
GAGCTTCACCGAAAGATTGGATTACTGGTCAATAATCGCCGTGGCAAATCGGTCAACGCCTTTATCTGTGACGCCGTCCG
GGAAAAACTGAGTCGTATTGACACTGAAGATACCCTCCACCATCACCGGTAG

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9