Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1045811..1046465 Replicon chromosome
Accession NZ_CP069980
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K21_RS04675 Protein ID WP_000244781.1
Coordinates 1045811..1046218 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K21_RS04680 Protein ID WP_000354046.1
Coordinates 1046199..1046465 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS04655 (1041768) 1041768..1043501 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
I6K21_RS04660 (1043507) 1043507..1044217 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K21_RS04665 (1044242) 1044242..1045138 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
I6K21_RS04670 (1045250) 1045250..1045771 + 522 WP_001055874.1 flavodoxin FldB -
I6K21_RS04675 (1045811) 1045811..1046218 - 408 WP_000244781.1 protein YgfX Toxin
I6K21_RS04680 (1046199) 1046199..1046465 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K21_RS04685 (1046708) 1046708..1047688 + 981 WP_000886087.1 tRNA-modifying protein YgfZ -
I6K21_RS04690 (1047765) 1047765..1048424 - 660 WP_000250274.1 hemolysin III family protein -
I6K21_RS04695 (1048588) 1048588..1048899 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K21_RS04700 (1048944) 1048944..1050377 + 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192462 WP_000244781.1 NZ_CP069980:c1046218-1045811 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192462 NZ_CP092417:c709789-709466 [Endozoicomonas sp. SCSIO W0465]
ATGAAGCGGGGCGACATCGTTACCGTCTCAATCAAGGGAGACTATGGCAAACCAAGGCCTGCCTTAATCATCCAGTCCAA
CCTGTTTCCGGATCACCCTTCGATAACTATCCTGCCAATCACAGGAGAGTTGAGGGATGTACCCCTATTCCGCTACACCC
TGTTTGAAAGTAAAGACAACGGACTCAACAAAACCTCCCAGGTGATGATCGATAAAATCACCACGATAAAAGCCGACAAC
ATCGGCAAAACCATCGGCTCACTGAGTCAACAAGAACTGACAGAAGTCACCCGACTGATGGCGTTATGGTTGGGAATGGC
CTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192462 WP_000354046.1 NZ_CP069980:c1046465-1046199 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192462 NZ_CP092417:c710016-709786 [Endozoicomonas sp. SCSIO W0465]
ATGTCTACCAATGCCAGCCAACCAAAAACCACAAGGGAACGTGTAGAAAGCCACAGAGAAGCACTGCGTCAGGCCGGTTT
GCGCCCTTTGCAGATTTGGGTTCCAGATACGCGGGTCAAGGGCTTCGATCTGGAGTGTCAGCGACAATCAGCCATGCTCA
AATCTGATAAACTGGAACGTGACACCATGGACTGGATTGAACAAACCAGCGACGATGAGGGCTGGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References