Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4913383..4913966 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6K29_RS23505 Protein ID WP_000254750.1
Coordinates 4913631..4913966 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6K29_RS23500 Protein ID WP_000581937.1
Coordinates 4913383..4913631 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS23490 (4909722) 4909722..4911023 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
I6K29_RS23495 (4911071) 4911071..4913305 + 2235 WP_000226815.1 GTP pyrophosphokinase -
I6K29_RS23500 (4913383) 4913383..4913631 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6K29_RS23505 (4913631) 4913631..4913966 + 336 WP_000254750.1 endoribonuclease MazF Toxin
I6K29_RS23510 (4914038) 4914038..4914829 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I6K29_RS23515 (4915057) 4915057..4916694 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I6K29_RS23520 (4916782) 4916782..4918080 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T192453 WP_000254750.1 NZ_CP069978:4913631-4913966 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T192453 NZ_CP092408:13774-14190 [Klebsiella quasipneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT192453 WP_000581937.1 NZ_CP069978:4913383-4913631 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT192453 NZ_CP092408:13547-13777 [Klebsiella quasipneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References