Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3354867..3355364 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K29_RS16235 Protein ID WP_001173343.1
Coordinates 3354867..3355040 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6K29_RS16240 Protein ID WP_001312331.1
Coordinates 3355068..3355364 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS16225 (3351537) 3351537..3352994 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
I6K29_RS16230 (3353231) 3353231..3354748 + 1518 WP_000003806.1 lysine--tRNA ligase -
I6K29_RS16235 (3354867) 3354867..3355040 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K29_RS16240 (3355068) 3355068..3355364 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K29_RS16245 (3355593) 3355593..3355865 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K29_RS16250 (3355877) 3355877..3356107 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
I6K29_RS16255 (3356288) 3356288..3357919 + 1632 WP_001216482.1 sensor histidine kinase -
I6K29_RS16260 (3357916) 3357916..3358635 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192450 WP_001173343.1 NZ_CP069978:c3355040-3354867 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192450 NZ_CP092406:c6279-6004 [Klebsiella pneumoniae]
TTGTCGAAGTGGAATATTAAGTTCACGAAGAATGGCGCTGATGATATCAATTACTGGCGTGGTACTGACAAAAAGAAGTT
TGAACGTATCCGGCAGCTACTTCACAGCATCGAGTCTGATCCGTTAACTGGTATAGGGAAGCCTGAGCGATTACGCCATC
ACAAAGATCCTGCGCTCTATTCTCGGCGCATCGATCAGAGCCATAGGCTTGTTTATTCAATCCAAAACGGAGAAATTATC
ATCTACGCAGCGCGTTATCACTACGGCGATAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT192450 WP_001312331.1 NZ_CP069978:c3355364-3355068 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192450 NZ_CP092406:c6505-6260 [Klebsiella pneumoniae]
ATGCATGCAATCACTTTTACGGATGCCCGTAAACATTTTGCCGATACCATGAAGCGTGTTACCGATGATGCTGAGCCGGT
ACGAGTAATGCGGCGAGATGCACCTGATATCGTCATGATCGATGCTGGAGAGTATGAGGCACTCATAGAAACAGTGTATC
TGTTCAGCAATCCTGCGAATGCTGCTCATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAATTTGTCGAAGTGGAA
TATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References