Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2455509..2456127 Replicon chromosome
Accession NZ_CP069978
Organism Escherichia coli strain FDAARGOS_1299

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K29_RS12005 Protein ID WP_001291435.1
Coordinates 2455909..2456127 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K29_RS12000 Protein ID WP_000344800.1
Coordinates 2455509..2455883 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K29_RS11990 (2450599) 2450599..2451792 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K29_RS11995 (2451815) 2451815..2454964 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K29_RS12000 (2455509) 2455509..2455883 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K29_RS12005 (2455909) 2455909..2456127 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K29_RS12010 (2456301) 2456301..2456852 + 552 WP_000102543.1 maltose O-acetyltransferase -
I6K29_RS12015 (2456968) 2456968..2457438 + 471 WP_000136192.1 YlaC family protein -
I6K29_RS12020 (2457602) 2457602..2459152 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K29_RS12025 (2459194) 2459194..2459547 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K29_RS12035 (2459926) 2459926..2460237 + 312 WP_000409908.1 MGMT family protein -
I6K29_RS12040 (2460268) 2460268..2460840 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192445 WP_001291435.1 NZ_CP069978:2455909-2456127 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192445 NZ_CP092400:190206-190622 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192445 WP_000344800.1 NZ_CP069978:2455509-2455883 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192445 NZ_CP092400:189979-190209 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGATGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References