Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 3429..4030 Replicon plasmid unnamed4
Accession NZ_CP069974
Organism Escherichia coli strain FDAARGOS_1280

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag I6K10_RS25750 Protein ID WP_001216034.1
Coordinates 3650..4030 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6K10_RS25745 Protein ID WP_001190712.1
Coordinates 3429..3650 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K10_RS25735 (422) 422..1693 - 1272 WP_023142242.1 restriction endonuclease subunit S -
I6K10_RS25740 (1690) 1690..3246 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
I6K10_RS25745 (3429) 3429..3650 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6K10_RS25750 (3650) 3650..4030 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6K10_RS25755 (4035) 4035..4214 + 180 WP_001513661.1 hypothetical protein -
I6K10_RS25760 (4242) 4242..4520 + 279 Protein_6 pdcB -
I6K10_RS25765 (4525) 4525..4938 + 414 Protein_7 integrase core domain-containing protein -
I6K10_RS25770 (5734) 5734..6711 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
I6K10_RS25775 (6996) 6996..7736 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
I6K10_RS25780 (7857) 7857..8039 - 183 WP_042065278.1 hypothetical protein -
I6K10_RS27160 (8106) 8106..8395 - 290 Protein_11 type II toxin-antitoxin system RelE/ParE family toxin -
I6K10_RS25785 (8382) 8382..8650 - 269 Protein_12 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / dfrA17 / aadA5 / qacE / sul1 / blaTEM-1B / blaCTX-M-15 / qnrS1 / erm(B) / mph(A) / aac(3)-IId - 1..136429 136429
- flank IS/Tn - - 4564..4938 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T192423 WP_001216034.1 NZ_CP069974:3650-4030 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T192423 NZ_CP092381:c3593198-3592947 [Xanthomonas oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT192423 WP_001190712.1 NZ_CP069974:3429-3650 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT192423 NZ_CP092381:c3593503-3593225 [Xanthomonas oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References