Detailed information of TA system TA192412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3788242..3788880
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: I6K10_RS19045
• Protein ID: WP_001306887.1
• Coordinates: 3788704..3788880 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K10_RS19040
• Protein ID: WP_001270286.1
• Coordinates: 3788242..3788658 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS19020 (3783394)	3783394..3784335	-	942	WP_001251315.1	ABC transporter permease	-
I6K10_RS19025 (3784336)	3784336..3785349	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
I6K10_RS19030 (3785367)	3785367..3786512	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
I6K10_RS19035 (3786757)	3786757..3788163	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
I6K10_RS19040 (3788242)	3788242..3788658	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K10_RS19045 (3788704)	3788704..3788880	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K10_RS19050 (3789102)	3789102..3789332	+	231	WP_000494241.1	YncJ family protein	-
I6K10_RS19055 (3789424)	3789424..3791385	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K10_RS19060 (3791458)	3791458..3791994	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
I6K10_RS19065 (3792086)	3792086..3793258	+	1173	WP_001523225.1	BenE family transporter YdcO	-
I6K10_RS19070 (3793366)	3793366..3793824	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3793366..3793824	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T192412 WP_001306887.1 NZ_CP069973:c3788880-3788704 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T192412 NZ_CP092379:4281798-4282079 [Xanthomonas oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT192412 WP_001270286.1 NZ_CP069973:c3788658-3788242 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192412 NZ_CP092379:4282097-4282387 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76