Detailed information of TA system TA192404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3262118..3262902
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K10_RS16355
• Protein ID: WP_000613626.1
• Coordinates: 3262118..3262612 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K10_RS16360
• Protein ID: WP_001110447.1
• Coordinates: 3262609..3262902 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS16335 (3257311)	3257311..3258408	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
I6K10_RS16340 (3258408)	3258408..3259349	+	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K10_RS16345 (3259415)	3259415..3261058	+	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I6K10_RS16350 (3261070)	3261070..3262023	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K10_RS16355 (3262118)	3262118..3262612	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K10_RS16360 (3262609)	3262609..3262902	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K10_RS16365 (3263035)	3263035..3266220	-	3186	WP_001522867.1	ribonuclease E	-
I6K10_RS16370 (3266793)	3266793..3267752	+	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192404 WP_000613626.1 NZ_CP069973:c3262612-3262118 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192404 NZ_CP092373:3373323-3373655 [Pantoea sp. Z09]
ATGGAGCGCGGCGAAATCTGGCTGGTCTCACTTGATCCCACTGCCGGTCATGAGCAACAGGGCACGCGGCCAGTATTAAT
CGTTTCGCCTGCGGCCTTCAACCAGCTAACCCAAACGCCTGTCGTACTGCCTGTCACAACCGGCGGGAATTTCGCTCGTA
CTGCGGGATTTACTGTTTCGCTTGATACGGCAGGAACGCGGACGCGTGGTGTTATTCGCTGCGATCAGCCGCGTGCAATT
GATATGCGCGCGCGTCATGGTAAACGGCTGGAGCACATTCCAGCCGACTTGATGGCAGAGGTGCAGGCGATTTTGCGTGC
GATACTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192404 WP_001110447.1 NZ_CP069973:c3262902-3262609 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192404 NZ_CP092373:3373066-3373323 [Pantoea sp. Z09]
ATGCACACGACTCATCTCCGTAAAGTGGGCGGTTCCGTCATGCTGGCTGTTCCGCCTGCTCTGCTTGATATTCTTCAACT
CAGTGCCGACGCACAGGTTGGCCTGACGGTCGATAACGGACGTCTGATTATTGAACCTAAAACTGTACCCAGTTATACGC
TGGCTGAGTTGCTGGCTAAATGTGATCCAGAAGCGGAACTGACTGCTGAAGATCGGCTGTGGATTGATGCCGTACCGGTG
GGGAAAGAGGAACTGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6