Detailed information of TA system TA192403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2973137..2973842
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K10_RS14935
• Protein ID: WP_000539521.1
• Coordinates: 2973456..2973842 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K10_RS14930
• Protein ID: WP_001280945.1
• Coordinates: 2973137..2973466 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS14915 (2968294)	2968294..2969205	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
I6K10_RS14920 (2969383)	2969383..2971731	+	2349	WP_021523051.1	EAL domain-containing protein	-
I6K10_RS14925 (2971739)	2971739..2973067	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
I6K10_RS14930 (2973137)	2973137..2973466	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K10_RS14935 (2973456)	2973456..2973842	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K10_RS14940 (2974068)	2974068..2975393	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K10_RS14945 (2975606)	2975606..2975989	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K10_RS14950 (2976100)	2976100..2977215	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I6K10_RS14955 (2977212)	2977212..2977838	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192403 WP_000539521.1 NZ_CP069973:c2973842-2973456 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192403 NZ_CP092373:2871739-2871957 [Pantoea sp. Z09]
ATGAACGATAAAATGTTGACGAAAACCGATTATTTGATGCGGCTGAGACGCTGCCGCTCAATCGACACGCTGGAGCGCGT
GATCGAAAAAAATAAGTATGAACTTTCTGATGATGAACTGGCGGTATTCTATTCAGCTGCCGATCACCGTCTGGCTGAAC
TGACGATGAATAAGCTTTACGACAAAGTGCCCGGCTCGGTATGGAAGTTTGTGCGTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192403 WP_001280945.1 NZ_CP069973:c2973466-2973137 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192403 NZ_CP092373:2871338-2871715 [Pantoea sp. Z09]
ATGGACGAGTACTCACCGAAACGGCATGATATTGCCCAGCTTAAATTCTTGTGTGAGAGCCTGTTTGATGAAAGTATGGC
GACGCTGACCGACAGCCACCATGGCTGGGTTAACGACCCGACTTCAGAGAGCAATTTACAGCTTAATGATTTGATTGAGC
ACATCGCTTCTTTCACGATGAATTACAAAATAAAGCATGCTGAAGATGAAGCCCTGATTACGCAAATCGACGACTATCTT
GATGATACCTTTATGCTGTTTACTAATTACGGTATCAATGCACAAGATTTGAATCGGTGGCAGCGTTCCGCCCGGCGCTT
ATTTAATATTTTTAGCGAAGAGTGTGCTTACCTTCAGCAACCCAGTCACTCCATTTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6