Detailed information of TA system TA192401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2352020..2353709
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: I6K10_RS12150
• Protein ID: WP_001521912.1
• Coordinates: 2352789..2353709 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: I6K10_RS12145
• Protein ID: WP_001521911.1
• Coordinates: 2352020..2352802 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS12120 (2347329)	2347329..2347487	-	159	Protein_2189	integrase	-
I6K10_RS12125 (2347579)	2347579..2347681	-	103	Protein_2190	helix-turn-helix domain-containing protein	-
I6K10_RS12130 (2347678)	2347678..2348148	-	471	WP_000201185.1	hypothetical protein	-
I6K10_RS12140 (2348495)	2348495..2351704	-	3210	WP_001521910.1	RNA-directed DNA polymerase	-
I6K10_RS12145 (2352020)	2352020..2352802	+	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I6K10_RS12150 (2352789)	2352789..2353709	+	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I6K10_RS12155 (2354143)	2354143..2355027	-	885	WP_001119037.1	LysR family transcriptional regulator	-
I6K10_RS12160 (2355292)	2355292..2356350	+	1059	WP_122452191.1	alpha/beta hydrolase	-
I6K10_RS12165 (2356492)	2356492..2357619	+	1128	WP_001521913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T192401 WP_001521912.1 NZ_CP069973:2352789-2353709 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T192401 NZ_CP092373:1506065-1506556 [Pantoea sp. Z09]
GTGATATCTGCCCCTGAGCCGCTGCATGCCGACCATGGGCTTTCTCTCTTTTGTTGCGGTGTGGAGTCGATGGACAACTG
GCTAAAACAGCGGGCCATGAAAAATCAGCTGACCGGCGCATCCCGAACATTTGTTTGCTGCGATGGCGCTAACGTGCTGG
CGTACTATTCTCTGGCATCCAGCGCAGTGGTGACAAGCGTCGCCCCAGGGCGTTTTCGCCGTAATATGCCCGATCCGATC
CCTGTGGTGGTATTGGGACGACTGGCCGTGGATAAAAGGATTCAGGGTCAGGGAGTGGCAAGGGCGCTGGTGCGCGATGC
AGGGTTGAGAGTCATTCAGGCATCAGACACTATCGGTATTCGCGGCATGCTGGTTCATGCCCTCTCTGATGAGGCGCGGC
GTTTTTATCTGCGGATCGGATTTGAACCTTCGCCGATCGATCCTATGGTGTTGATGGTGACGCTGGGAGACGTTATGCAG
ACGCTGCTCTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT192401 WP_001521911.1 NZ_CP069973:2352020-2352802 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT192401 NZ_CP092373:1505775-1506068 [Pantoea sp. Z09]
ATGCCAGCGCCAGACAGCTTTTCAGTGAAACGCGAGACGCTCAATTTACGCATCAGGCCTGCGGAACGAGACCTTATCGA
TCGCGCAGCAAAGGCGAAAGGAAAAAACCGTACCGATTTCGTTCTCGATGCGGCTCGGGCCGCCGCTGAAGACGCCTTAA
TTGAGCAGCGCGTGATGCTGGTCGATCCCGACGGCTGGCAGGCGTTTATCACCCGCCTGGACCAGCCTCCCGCGCCCAAC
GCTGAACTGCGTCGCACCATGCAAACCCCCGCGCCCTGGGACGCCAAAAAGTGA

Structures

Toxin

• AlphaFold DB: A0A829L165