Detailed information of TA system TA192399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1944765..1945579
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: I6K10_RS10250
• Protein ID: WP_001054376.1
• Coordinates: 1945322..1945579 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: I6K10_RS10245
• Protein ID: WP_001309181.1
• Coordinates: 1944765..1945310 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS10215 (1940456)	1940456..1941769	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
I6K10_RS10220 (1941781)	1941781..1942059	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
I6K10_RS10225 (1942056)	1942056..1943177	-	1122	WP_000010829.1	M42 family metallopeptidase	-
I6K10_RS10230 (1943422)	1943422..1943538	-	117	Protein_1818	VOC family protein	-
I6K10_RS10235 (1943576)	1943576..1943794	-	219	Protein_1819	hypothetical protein	-
I6K10_RS10240 (1943963)	1943963..1944709	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
I6K10_RS10245 (1944765)	1944765..1945310	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
I6K10_RS10250 (1945322)	1945322..1945579	-	258	WP_001054376.1	YjhX family toxin	Toxin
I6K10_RS27015 (1946070)	1946070..1946201	-	132	WP_001309182.1	hypothetical protein	-
I6K10_RS10260 (1946317)	1946317..1947557	+	1241	Protein_1824	helicase YjhR	-
I6K10_RS27020 (1947825)	1947825..1948030	-	206	Protein_1825	HNH endonuclease	-
I6K10_RS10265 (1948140)	1948140..1949120	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
I6K10_RS10270 (1949185)	1949185..1950291	-	1107	WP_204638466.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T192399 WP_001054376.1 NZ_CP069973:c1945579-1945322 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T192399 NZ_CP092373:c1286740-1286342 [Pantoea sp. Z09]
ATGCATCGTTATATGCTGGACACCAACATTGTTATCTATGTGATAAAGCGGCGGCCCATAGAGATGCTGAGCAAGTTCAA
CGCGCAACTGGGGCGCATGGTGATCTCCTCCATTACGCTGGCGGAACTCTATTACGGCGCCGAGAAAAGCCAGTTCCCCG
ATCGCAATCTGCGCGTCGTTGAGGATTTCGTGTCTCGTCTCGACGTGCTGAGCTATGACGCCAGAGCTGCCTTTCACTAC
GGCGCCATTCGCGCCGATCTTGAGCGTAAAGGGACACCAGTCGGGGCGAACGATCTGCATATCGCCGCGCATGCGCGCAG
CATGGGCCTGACGCTGGTGTCAAATAACCTACGCGAATTCAATCGGGTGGAAGGTTTGCTCAGCGAAAACTGGCTTTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT192399 WP_001309181.1 NZ_CP069973:c1945310-1944765 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT192399 NZ_CP092373:c1286973-1286740 [Pantoea sp. Z09]
ATGTCGCAAGGCACTATTTTTACCAGCAATCGCACGCAAAACGTACGTATTCCCGCCGATGTGCGTTTTCCGGAAAACGT
GAAAAACGTGACCGTCCGGGCGGTGGGCAATGAACGCATTATCGCGCCGGTCGAGCATGCCTGGGACAGTTTTTTCCTGA
GCGAACAGTGCGTCAGCGACGACTTTCTGTCAGAGCGAACCACGCCGCCAGCTGATGAACGGGAAGCGTTGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8