Detailed information of TA system TA192397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1829611..1830206
• Replicon: chromosome
• Accession: NZ_CP069973
• Organism: Escherichia coli strain FDAARGOS_1280

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: I6K10_RS09680
• Protein ID: WP_000239581.1
• Coordinates: 1829856..1830206 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: I6K10_RS09675
• Protein ID: WP_001223213.1
• Coordinates: 1829611..1829862 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K10_RS09665 (1825276)	1825276..1829055	+	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-
I6K10_RS09670 (1829058)	1829058..1829399	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
I6K10_RS09675 (1829611)	1829611..1829862	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
I6K10_RS09680 (1829856)	1829856..1830206	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
I6K10_RS09685 (1830285)	1830285..1830815	-	531	WP_000055075.1	inorganic diphosphatase	-
I6K10_RS09690 (1831125)	1831125..1832081	+	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
I6K10_RS09695 (1832214)	1832214..1833716	+	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
I6K10_RS09700 (1833730)	1833730..1834752	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T192397 WP_000239581.1 NZ_CP069973:1829856-1830206 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T192397 NZ_CP092373:c152875-152348 [Pantoea sp. Z09]
ATGGAAGCGGTAACATTAAAAATCATCAGGTTTTCGCCGCAGCTGCCTCTCAATAGCGCGAATTTTAGCTGTGGTCGGCC
CGCGTTAGACGACTTCCTGAAAAAACATCTGGCCGCGCAGCACCAGCAGAACGTCCTTAAGGCGTATCTGCTGGTCACCG
ACGATCCTGTTGCAGAAGTGCTGGGTTATTACACACTGTCAGGCGGCAGTTACGCCAGAAGCGCCATGACGCGCACCTTT
CAAAAACAGGCTGCGCCCTACCATGAAACCGCCTGTATCATTCTTAGCCGTCTGGCGGTCGATAAGCGCATTGCCGGCAA
GGGCTTCGGCACGCAGCTGGTGCTGGATGCGCTGCGCGTCGCCTGGCACGCGGCGGATGCCGTCGGCATTTACGCCGTCA
TGGTGGAAGCCAAAGATGAAGAGGCCGCCGGTTTTTATCGCGAAGCGATGAATTTTATCCCGCTCAAAACCGACGACGGG
AAACTGAAATTCTTTTTTCCCGTCGCAAGCATCGCGTCGCTACTGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT192397 WP_001223213.1 NZ_CP069973:1829611-1829862 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT192397 NZ_CP092373:c153153-152863 [Pantoea sp. Z09]
ATGGGCGCTTCAGCTAAATCGGAACGGATGGAACTGCGGATAAGCCCGCATGACAAGCAAGAGATCGAACAGGCTGCGCA
GTTGAGCCGCGTCAGCGTGTCGCAGTTTATTGCCGACTCGGCGCGAGAGCGTGCGGAACAGATTATCAATGAACACAAGC
GCATCCAGCTGACCGAAGCCTCCTATAGAGCGGTCATGGAGGCGCTGGAAAACCCGCCGGAACCGAATGAACGACTCCGT
CGCGCAGCGGATCTCAGCAGAAAGGATTCAACCTGGGAATGGAAGCGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7