Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1657264..1657761 Replicon chromosome
Accession NZ_CP069973
Organism Escherichia coli strain FDAARGOS_1280

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K10_RS08775 Protein ID WP_001173343.1
Coordinates 1657588..1657761 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K10_RS08770 Protein ID WP_000398619.1
Coordinates 1657264..1657560 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K10_RS08750 (1653993) 1653993..1654712 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6K10_RS08755 (1654709) 1654709..1656340 - 1632 WP_001216477.1 sensor histidine kinase -
I6K10_RS08760 (1656521) 1656521..1656751 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K10_RS08765 (1656763) 1656763..1657035 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K10_RS08770 (1657264) 1657264..1657560 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K10_RS08775 (1657588) 1657588..1657761 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K10_RS08780 (1657880) 1657880..1659397 - 1518 WP_000003806.1 lysine--tRNA ligase -
I6K10_RS08785 (1659634) 1659634..1661091 - 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192395 WP_001173343.1 NZ_CP069973:1657588-1657761 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192395 NZ_CP092370:c235428-235129 [Flavobacterium sp. CBA20B-1]
ATGGGGTATAAAATCAGTCATGAAGCGAATCGGGATATTGAGAACATTTGGCTTTATACATATGAAAACTGGTCGCTCGA
ACAAGCGGATAGATACATCAACCTGATAATTGACGAAATAGAATACTTAGCGGAAAATCCAAAATCAGGAAAAGATTATG
GGCATCTAAGAAAAGGCTATTTTCGTTCTCAAATAAAATCACATTTCATTTTCTATAAAATCAGTCTAAAAAGAGAAGAA
ATTGAAATCATCAGAGTTTTGCATCAAAGAATGGATATTGAATCCCGACTGACTGAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT192395 WP_000398619.1 NZ_CP069973:1657264-1657560 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192395 NZ_CP092370:c235676-235428 [Flavobacterium sp. CBA20B-1]
ATGGCTAAAAACACATCTATTTTGCTCGGGGATTATTTTGACAATTTTATCAGCCGACAAATTAAAACAGGAAAATATTC
TTCCGCAAGTGAAGTTGTTAGAGCGGCTTTGAGAATGTTCGAGTACGAAGAGTCAAAAAGAACCGAACTGATAAAGGAAC
TGAAAAAAGGAGAAAAATCAGGTTTTGTAAAAAACTTTGACAGAAAAGAACTTTTAAATAGCCTTCATCAAAAGCATTTG
AGTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References