Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 14181..14782 Replicon plasmid unnamed1
Accession NZ_CP069970
Organism Escherichia coli strain FDAARGOS_1280

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag I6K10_RS00060 Protein ID WP_001216045.1
Coordinates 14402..14782 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6K10_RS00055 Protein ID WP_001190712.1
Coordinates 14181..14402 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K10_RS00045 (I6K10_00045) 11189..12445 - 1257 WP_072196859.1 restriction endonuclease subunit S -
I6K10_RS00050 (I6K10_00050) 12442..13998 - 1557 WP_060578235.1 type I restriction-modification system subunit M -
I6K10_RS00055 (I6K10_00055) 14181..14402 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6K10_RS00060 (I6K10_00060) 14402..14782 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6K10_RS00065 (I6K10_00065) 14787..14966 + 180 WP_000113018.1 hypothetical protein -
I6K10_RS00070 (I6K10_00070) 14994..16037 + 1044 WP_029393260.1 DUF968 domain-containing protein -
I6K10_RS00075 (I6K10_00075) 16126..16578 + 453 WP_001326849.1 late promoter-activating protein -
I6K10_RS00080 (I6K10_00080) 16664..17857 + 1194 WP_060578234.1 hypothetical protein -
I6K10_RS00085 (I6K10_00085) 17899..19341 + 1443 WP_223656860.1 terminase -
I6K10_RS26905 19555..19656 + 102 Protein_17 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..39407 39407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T192382 WP_001216045.1 NZ_CP069970:14402-14782 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T192382 NZ_CP092362:c5033638-5033192 [Mycolicibacterium crocinum]
ATGGCGAAACTCTCAGTCTCCGTTGACGTTCCACTTCCACCAGAGCAGGCCTGGCAGCACGCCTCGGACCTCTCCCGCTA
CAAGGACTGGCTGACCATCCACCGGGTGTGGCGCTCCAAATTGCCCGACACCCTCGAGAAGGGCACCGTCGTGGAGTCCA
TCGTCGAGGTCAAGGGCATGCCCAACCGGGTGAAGTGGGAGATCGTCCACTTCAAGGCCCCCGAGGCCATGACGCTCAAT
GGCGCCGGTGTCGGCGGGGTGAAGGTCAAGCTGATCGGCAAGGTGCGGGCCCACGACAAGGACGCAGGCAGTTCGGTGGT
GACCATGGACGTCCATCTGGGCGGTGCGGCGCTGTTCGGGCCGATCGGCATGGTCGTGGCGGGTGCACTCAAGGGCGACA
TCAAGGAGTCGCTGAACCGCTTTGTGACGGTATTCGCGCCGTCGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT192382 WP_001190712.1 NZ_CP069970:14181-14402 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT192382 NZ_CP092362:c5033848-5033642 [Mycolicibacterium crocinum]
ATGGGATTCCTCGACAAAGCCCTCGGCAAGACCAAGGAAGTTCTGGGCTCCAACGCAGACAAGGTGGAGCAGGCCATCGA
TAAGGCCGGCGACATCATCGACCAGAAGACGCAGGGCAAGTACTCCAGCACCGTCGACAGGGTGCAGGACGCCGCCAAGA
ACTACGTCGACTCCAACCGCACCGACAAAGGCGAGCAACCCAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References