Detailed information of TA system TA192361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 30113..30756
• Replicon: plasmid unnamed1
• Accession: NZ_CP069967
• Organism: Klebsiella pneumoniae strain FDAARGOS_1308

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: I6K38_RS00155
• Protein ID: WP_114690528.1
• Coordinates: 30340..30756 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: I6K38_RS00150
• Protein ID: WP_001261276.1
• Coordinates: 30113..30343 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K38_RS00125	26235..26861	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
I6K38_RS00130	26981..27160	+	180	WP_006788217.1	hypothetical protein	-
I6K38_RS00135	27608..28399	-	792	WP_032667567.1	site-specific integrase	-
I6K38_RS00140	28396..29139	-	744	WP_004098973.1	hypothetical protein	-
I6K38_RS00145	29190..29540	-	351	WP_000493378.1	hypothetical protein	-
I6K38_RS00150	30113..30343	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6K38_RS00155	30340..30756	+	417	WP_114690528.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6K38_RS00160	31107..32075	-	969	WP_084832609.1	IS5 family transposase	-
I6K38_RS00165	32109..32246	-	138	Protein_32	sugar dehydrogenase	-
I6K38_RS00170	32312..33013	-	702	WP_008502228.1	DsbA family oxidoreductase	-
I6K38_RS00175	33079..34185	-	1107	WP_006687059.1	alkene reductase	-
I6K38_RS00180	34398..34727	+	330	WP_087833142.1	thioredoxin family protein	-
I6K38_RS00185	34757..35095	-	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
I6K38_RS00190	35100..35681	-	582	WP_008502230.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(6)-Id / qnrB4 / sul1 / blaDHA-1	-	1..48813	48813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15181.66 Da
• Isoelectric Point: 8.5404

>T192361 WP_114690528.1 NZ_CP069967:30340-30756 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T192361 NZ_CP092346:1603804-1604310 [Rhizobium gallicum]
ATGCCTGAGACCGTCGTGCATCTATGGCGCGCCTTCGTTCCACGATGGGCCCATGCGCCGCTTTCTGGGGAGGGCGCCAG
CCGTTTTGGTGGCCGGTGGAATCGGGTGGGTGCCCCGACCATCTATGCGGCCTGCGAGCTGTCCACGGCCTGGGCCGAAT
ATAACCAGGGCTTTGTTCAGCACCCGGCGATGATCGTGCAATTGGAACTCAAAGGGGCGTGCCTCGCGGACACGACCGAC
CAGATGCTTCTTCGAAAGTTTGACGTTACTGCGGATATCCATCGTTGCGAATGGCGGATGCTGATGGATGAAAGCAAGGT
GCCGGCAACCCACCACCTGCGCGAACGTCTGATTGCATCGCGATATCATGGCGCGATCTATCCATCTTTCATGTCGCTCG
GCGGCACATGCGTCGCGCTGTGGGAATGGAATATGCCGGGTGCACCGGAGCTTCGCGCAATCGATCCAGAGGGTCGACTG
CCCAAATCACCGGCCTCCTGGTTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT192361 WP_001261276.1 NZ_CP069967:30113-30343 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT192361 NZ_CP092346:1603440-1603811 [Rhizobium gallicum]
ATGGCAGCCACAGGCCTCAGCAATGTCGCTGCTCGCTTCGGCGATGAACATTCGTCGTTTCTATCGGCCCGGCGCGTCGC
CGAGCAACTGGGCGTAACGCTCTCCGAGCTAGCCCGGCTGATCGGGGTTGCGCGCAATACGCTGGCGGCGAAATCGGGCG
CCCGCAAAGTGGATAATGCGCTGAGCAACGTCGTCCGCATCCTTGCGATGGCAAGCGAAATGGCCGGCGACGAAAACCGT
GCGGTCATATGGTTCAAGCATCAGCCGATCCCCGGTTGGGGCGGCAAGACGGCGTTTGACCTTGTCGGCGAAGGCAAGTC
CGACCGGGTTCTCGCCTATCTCGAGGCCGTCCGCTCCGGCGTTTATGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023