Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 133965..134701 Replicon plasmid unnamed2
Accession NZ_CP069964
Organism Klebsiella pneumoniae strain FDAARGOS_1307

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag I6K37_RS26955 Protein ID WP_003026803.1
Coordinates 134219..134701 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K37_RS26950 Protein ID WP_003026799.1
Coordinates 133965..134231 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K37_RS26905 130027..130389 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
I6K37_RS26910 130439..130789 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
I6K37_RS26915 131147..131416 + 270 WP_004152102.1 hypothetical protein -
I6K37_RS26920 131404..131979 + 576 WP_004152103.1 hypothetical protein -
I6K37_RS26925 132010..132504 + 495 WP_004152104.1 DNA-binding protein -
I6K37_RS26930 132548..132916 + 369 WP_004152105.1 hypothetical protein -
I6K37_RS26935 132950..133153 + 204 WP_004152106.1 hemolysin expression modulator Hha -
I6K37_RS26940 133202..133459 + 258 WP_004152107.1 hypothetical protein -
I6K37_RS26945 133535..133789 + 255 WP_004152108.1 hypothetical protein -
I6K37_RS26950 133965..134231 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K37_RS26955 134219..134701 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
I6K37_RS26960 134913..136259 + 1347 WP_020314316.1 ISNCY family transposase -
I6K37_RS26965 136760..138014 - 1255 Protein_144 IS3 family transposase -
I6K37_RS26970 138102..139064 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..232943 232943


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T192360 WP_003026803.1 NZ_CP069964:134219-134701 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T192360 NZ_CP092346:c1285536-1285318 [Rhizobium gallicum]
ATGAGACTAGTCTGGTCGCAATACGCTCTTGATGATCGCGACAGTATCTTCAGCTATATCGAAGCAGAAAACCCGCGCGC
GGCGGTTCATGTCGATGAAGAGGTCGTTCGCGCCGCGCGTCGTCTCCTTGAGTTTCCTGAAAGCGGACGTCCCGGCCGGA
TTGCCGGAACACGCGAACTTGTTTATCCCGCGCACACCTTATATTGCGCTATGCAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT192360 WP_003026799.1 NZ_CP069964:133965-134231 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192360 NZ_CP092346:c1285835-1285536 [Rhizobium gallicum]
ATGGCTTCAAACGCACTAGTTCAGACTCGGATTGACGCCGCCGTCAAGGAGCGCGCAACGGCGGTGCTGGAGAACATGGG
ACTGACAGTTTCGGATGCCGTGCGCATCCTATTGACCCGAACCGCAAACGAAGGCGCCTTGCCATTGGAGCTTGTCAGTA
ATAGCGACGTCCACGATGCCTGGTTCCGTGCTAAAGTACTGCAAGCGCTCGAGGATACGCGAGCCGACATCGAAGATGCC
GACGCCGATAAGCATTTCAGTGCACGCCGGTCAGCAGCACTGCTTAAGGGAAGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References