Detailed information of TA system TA192359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 45447..45973
• Replicon: plasmid unnamed2
• Accession: NZ_CP069964
• Organism: Klebsiella pneumoniae strain FDAARGOS_1307

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: I6K37_RS26515
• Protein ID: WP_000323025.1
• Coordinates: 45447..45734 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: I6K37_RS26520
• Protein ID: WP_000534858.1
• Coordinates: 45734..45973 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K37_RS26475	41053..41283	-	231	WP_020805755.1	hypothetical protein	-
I6K37_RS26480	41371..41718	-	348	WP_020316654.1	hypothetical protein	-
I6K37_RS26485	41784..42164	-	381	WP_015632488.1	hypothetical protein	-
I6K37_RS26490	42681..43031	-	351	WP_020277938.1	hypothetical protein	-
I6K37_RS26495	43028..43300	-	273	WP_023280873.1	hypothetical protein	-
I6K37_RS26500	43490..43975	+	486	WP_020277937.1	hypothetical protein	-
I6K37_RS26505	44136..44297	-	162	WP_032426744.1	type I toxin-antitoxin system Hok family toxin	-
I6K37_RS26510	44358..45392	+	1035	Protein_53	IS481 family transposase	-
I6K37_RS26515	45447..45734	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
I6K37_RS26520	45734..45973	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
I6K37_RS26525	46236..47159	-	924	WP_020277922.1	cation diffusion facilitator family transporter	-
I6K37_RS26530	47359..47931	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
I6K37_RS26535	48407..49645	-	1239	WP_003030308.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..232943	232943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T192359 WP_000323025.1 NZ_CP069964:c45734-45447 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T192359 NZ_CP092346:928577-929074 [Rhizobium gallicum]
GTGACCCTCAGCGCACCGACACCACTGGCCGACCATCACGAACTGGCGGAATTCATTTCCGGCGTCGTTGAACTGGACGA
TTGGCTGCGTCGCCGCGCCCGCGCCAATCAGGCAGGCGGCGCATCGCGCACATTCGTCGTGTGCGAGGGCAACCGCGTGA
TCGCCTATTATGCGCTTGCCTCCGGCGCCGTTAAGCAGCCGGAAGCACCTGGCCGTTTCCGACGCAACATGCCCGACCCG
ATTCCGGTTGCCGTTCTCGGCCGCCTCGCCATCGACCGGACCTTTCAGGGGCGCGGTCTCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGTCTGCTCAATGCCGCTGAAATCCTCGGTATCCGCGGCGTGCTGGTACATGCGATTTCCGATGATGCGC
GAGCCTTCTACGAGGCAGTCGGTTTTCTGCCTTCGCCGTCCGACCCCATGATGCTGATGGTTGGGCTGCGTGATTTGAAC
AACGCGCTCAATCCTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT192359 WP_000534858.1 NZ_CP069964:c45973-45734 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT192359 NZ_CP092346:928284-928580 [Rhizobium gallicum]
ATGCCCACATCCCACACCAGTAAAAGCGCAGATGCCAGCGTGCCGCTGAATATCCGCATCAAGCCGGCGACCCGAAACCT
TATCGATCGCGCCGCCGAGCTGCTTGGCAAGACGCGCACGGATTTCATGCTGGAGGCTTCCGAGCGCCGCGCCGAGGAAG
TTCTGCTCGACCGTACGATATTCACGGTTAGTCCCGAACTCTATGCCGAGTACCTTGCCCGGCTCGATGCGCCCGCGCAA
CCCAATGAGCGACTGAAACGTACTATGACGACCAAGGCGCCGTGGGATGAGGCGTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8