Detailed information of TA system TA192358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 53380..53873
• Replicon: plasmid unnamed1
• Accession: NZ_CP069963
• Organism: Klebsiella pneumoniae strain FDAARGOS_1307

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: I6K37_RS26175
• Protein ID: WP_001230709.1
• Coordinates: 53380..53634 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: I6K37_RS26180
• Protein ID: WP_000151456.1
• Coordinates: 53631..53873 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K37_RS26140	48952..49113	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
I6K37_RS26145	49263..49859	+	597	WP_000125165.1	hypothetical protein	-
I6K37_RS26150	49877..50161	-	285	WP_001162371.1	hypothetical protein	-
I6K37_RS26155	50235..50900	-	666	WP_000532148.1	P-loop NTPase	-
I6K37_RS26160	51489..51818	+	330	WP_000855119.1	hypothetical protein	-
I6K37_RS26165	52222..52737	+	516	WP_001025393.1	J domain-containing protein	-
I6K37_RS26170	52841..53383	+	543	WP_001083904.1	hypothetical protein	-
I6K37_RS26175	53380..53634	+	255	WP_001230709.1	hypothetical protein	Toxin
I6K37_RS26180	53631..53873	+	243	WP_000151456.1	hypothetical protein	Antitoxin
I6K37_RS26185	54444..54737	+	294	WP_000673428.1	hypothetical protein	-
I6K37_RS26190	55147..55329	-	183	WP_024135751.1	hypothetical protein	-
I6K37_RS26195	55887..56069	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
I6K37_RS26200	56094..56531	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
I6K37_RS26205	56662..56982	-	321	WP_001401701.1	hypothetical protein	-
I6K37_RS26210	56979..57494	-	516	WP_001229210.1	hypothetical protein	-
I6K37_RS26215	57496..57699	-	204	WP_000224735.1	hypothetical protein	-
I6K37_RS26220	57705..58154	-	450	WP_000206700.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-2	-	1..64998	64998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-64)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T192358 WP_001230709.1 NZ_CP069963:53380-53634 [Klebsiella pneumoniae]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T192358 NZ_CP092346:c816117-815695 [Rhizobium gallicum]
GTGAGACTGCTCCTCGACACCAATGTCTTGTCCGAGGTGACGAAGCCGTGTCCCGATACACGCGTCCTGGAATGGCTTGA
TGGGCTTGACGAAGATCGCGCCTTCATCAGTGTTGTATCGATCGCGGAAATTCGCCGCGGTGTTGCTCTGATGGACAGTG
GGCGAAAACGCGACGCGCTGGCGGAATGGCTTGCCAGGGATCTTCCGCAACGTTTCGAGAAGCGCGTCATTCCGGTAGAG
GAGCCTGTCGCCCTGGCGTGGGGCGAGCTGATGGCACTTGCGAAGCGAAGCGGTCGCGGGCTCTCATCGATGGACGGTTT
GATCGCTGCTACGGCAATGGCACACGATCTTACCCTCGCGACGCGCAATACCAGGGACTTTGAAGGTTTCGGAATCGAGA
TCGTCGATCCATGGAGCAGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT192358 WP_000151456.1 NZ_CP069963:53631-53873 [Klebsiella pneumoniae]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT192358 NZ_CP092346:c816380-816114 [Rhizobium gallicum]
ATGCAACCGTCAACGCACGATGACAAAAGCTGGACGGTCGCCAGTGCCAAGGCAAAACTTTCGGAGGTTATCGAACGCGC
ACAATCTGTGCCCCAGACGATCACCCGAAACGGAAAACCGAGCGTCGTCGTCGTGTCGGCCGAGGAATGGCACAAGAAGA
CCGCACGCAAAGGTTCGCTCGCCGAGTTCCTGATGGACTCGCCACTTCGCGGCGCCGATCTGGACCTCGAGCGTCAACGT
GATGAACCACGTGATTTGCCGCTGTGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5