Detailed information of TA system TA192330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4033939..4034529
• Replicon: chromosome
• Accession: NZ_CP069961
• Organism: Klebsiella pneumoniae strain FDAARGOS_1317

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: I6K47_RS19515
• Protein ID: WP_023341911.1
• Coordinates: 4034197..4034529 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: I6K47_RS19510
• Protein ID: WP_000288812.1
• Coordinates: 4033939..4034196 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K47_RS19490	4029764..4030087	-	324	WP_016532370.1	hypothetical protein	-
I6K47_RS19495	4030273..4032021	+	1749	WP_204779768.1	hypothetical protein	-
I6K47_RS19500	4032100..4032658	+	559	Protein_3788	hypothetical protein	-
I6K47_RS19505	4033398..4033604	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
I6K47_RS19510	4033939..4034196	+	258	WP_000288812.1	antitoxin	Antitoxin
I6K47_RS19515	4034197..4034529	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6K47_RS19525	4034851..4036287	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
I6K47_RS19535	4036653..4038107	-	1455	WP_004148975.1	AMP nucleosidase	-
I6K47_RS19540	4038237..4038482	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T192330 WP_023341911.1 NZ_CP069961:4034197-4034529 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T192330 NZ_CP092289:c2883766-2882453 [Marinobacter sp. M1C]
GTGAGCACCGTTACGGAAGTTATCCTATGGGGGAAGAGTGTCGGTGCCCTGCTGTATGACACAGAAACCGGTCTGTCGAC
GTTCGAATACGATAAGGGTTGGTTGAAGCAGGACGTTTCAATTTCCCCGCTCCACCTACCTCCTCGGGCTGGGAGGTTCA
ATTTCCCCTCATTGCCACAGGAAACGTTCTTTGGACTGCCGGGCGTGTTTGCCGACACTTTGCCAGATGACTTTGGCAAT
GCCGTTATCAACGCTTGGCTGGCTCGCCAGGGAATCCCAAAAAGCTCATTCAGCCCGGTTGACCGCCTCCTATACACCGG
CAACCGTGGCATGGGTGCGCTGGAGTACAAGCCAGACGGCCGGAACACTCAGACTCAGGTATCAGGAGATATCAAGGTCG
ATACACTGGTCGAGTTGGCTCAGTCCGTGTTGGACCAAAGAGCTGGCCTGGATGTGGCCATATCAGAGGGTGATGAGAAC
AATGATGCGATGAGTGCCATTTTCCAGGTGGGCACATCGGCGGGTGGGGCTCGTCCCAAGGCCGTTATTGCCGTGAACCG
TGATCGTTCTCGGGTAGTTTCCGGCCAGACTCCCATACCCAAAGGATTCACTCACTACCTCATTAAATTCGATGGGGTCA
AAGAGCGATCATCCACCAGCGAGGTCTTTGGTGACCCGCAGGGGTATGGCCGGATGGAATATGCCTACTACCTGATGGCC
AAGGACGCAGGCATCAATATGGCACCGTCTGAACTGTTGATAGAAGGGGAGCGAGCCCATTTTATGACCCAGCGTTTCGA
CCGGGACGGTGACCGCAAGCGCCACTACCAGTCCCTATGCGCGATGGACCATGCGGACTTCCGGAAACCTGGGCAGTACA
GCTATGAAGAACTGATGACCATTGCCCGAACACTCGGGTTGCCACGGGTAGACGCAATCGAGATTTTCAGGCGTATGGTC
TTCAACGTCATTGCCCGGAACCAGGACGACCACACCAAGAACTTTGGCTTTCTGTTGGATCATTCAACGGCCGGTTGGCG
TTTGGCACCGGCGTTTGATATCGCCTACAGCTACAAACCCGGTTCATACTGGGTGGACACCCATCAGATGACCATCAACG
GTAAACGGGATAAGTTCACTCGCGGGGATATTCTCGCAGTCGCAACCCAAATTCCCCGTTTCGAGTCAAATGCCCGGGCC
ATTATTGACCAGGTAACTGAGGTGGTGAACCAATGGCAGGATTATGCTCGCCGAGCGGATGTCCATTCGGAGCTGTCACA
GGAGATTTACTCCAACCTCCGGTTGTCCTTGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT192330 WP_000288812.1 NZ_CP069961:4033939-4034196 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT192330 NZ_CP092289:c2884104-2883763 [Marinobacter sp. M1C]
ATGAGCCAAATTAAGCGCAGCATGTCAGACTTGGCCATCCTCGATGAGCTGGGTCGGCGCCTCAAGCAAGCCCGACTGGA
GCAAAACATCACCCAGCAGCAGATCGCAGAGGGCATGGGTGTCAGCCGAGCAACGTACATCCGGCTTGAGGATGGTAACG
GGAAACTTGAAGTCCTGGTTGCTGCCCTGCGTGTTCTCGGAAAGCTCCCGGCGTTGGATGCCTTCTTGCCAGATGAGCCT
TATTCACCGATTGCGGCATTAAAGAGTGCTGGCCATATTCGCCAGCGTGCACGCCCCGGTAATAAGCCCGTTGAACAGGA
CAAAGGAGACCTGGGCTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0