Detailed information of TA system TA192323

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 1643856..1644544
• Replicon: chromosome
• Accession: NZ_CP069961
• Organism: Klebsiella pneumoniae strain FDAARGOS_1317

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: I6K47_RS08025
• Protein ID: WP_023328858.1
• Coordinates: 1643856..1644197 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K47_RS08030
• Protein ID: WP_023328857.1
• Coordinates: 1644218..1644544 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K47_RS07995	1639076..1639750	+	675	WP_004152211.1	hypothetical protein	-
I6K47_RS08000	1639752..1640099	+	348	WP_004199257.1	hypothetical protein	-
I6K47_RS08005	1640102..1640581	+	480	WP_002887049.1	type VI secretion system tube protein Hcp	-
I6K47_RS08010	1640773..1641096	-	324	Protein_1559	hypothetical protein	-
I6K47_RS08015	1641115..1642226	-	1112	Protein_1560	IS3 family transposase	-
I6K47_RS08020	1642594..1643601	-	1008	WP_023328859.1	restriction endonuclease	-
I6K47_RS08025	1643856..1644197	-	342	WP_023328858.1	TA system toxin CbtA family protein	Toxin
I6K47_RS08030	1644218..1644544	-	327	WP_023328857.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K47_RS08035	1644558..1645034	-	477	WP_023328856.1	DNA repair protein RadC	-
I6K47_RS08040	1645045..1645485	-	441	WP_023328855.1	antirestriction protein	-
I6K47_RS08045	1645555..1645785	-	231	WP_023328854.1	DUF905 domain-containing protein	-
I6K47_RS08050	1645809..1646636	-	828	WP_032430976.1	DUF945 domain-containing protein	-
I6K47_RS08055	1646732..1647409	-	678	WP_023328852.1	hypothetical protein	-
I6K47_RS08060	1647695..1648588	-	894	WP_023328851.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1639752..1674714	34962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12806.78 Da
• Isoelectric Point: 9.8952

>T192323 WP_023328858.1 NZ_CP069961:c1644197-1643856 [Klebsiella pneumoniae]
MKTLPATTPQAAKLYLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T192323 NZ_CP092289:c1045331-1045059 [Marinobacter sp. M1C]
GTGCTAACGCCAGTGCAATCGACCCAGTTCAAAAAAGACGTGAAGAAAGCCCGCAAGCGGGGCAAAGACTTGGACAAACT
AAAGATGTCGCTCAGTTTGCTGATTCGCCAGGCACCGTTACCCGAAGCTTATCAAGATCATCCTTTACGGGGGAACTGGA
AGGGCTATCGAGACGCGCATATTGAGCCGGACTGGTTGCTTTTGTACCGAGTGACCGACAAGGAATTGCAGTTAGCCCGA
ACCGGAACGCACGCGGATTTGTTTCAGGAGTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12320.00 Da
• Isoelectric Point: 6.9461

>AT192323 WP_023328857.1 NZ_CP069961:c1644544-1644218 [Klebsiella pneumoniae]
MSSNDLQQWGLCRSISPCFGARLVQERNRLHFLADRAGFNGQFTEEQALRLDQAFPLIMKQLELMLSTGELNPRHSHCVT
LYHNGLTCEADTLGSYGYVYIAVYPTHR

Nucleotide

• Length: 327 bp

>AT192323 NZ_CP092289:c1045588-1045331 [Marinobacter sp. M1C]
ATGAATACCGATTCTGTAGTTCGTGCCCGCATCGACAGTGACACCAAAGCCCGCGCCACCGCCGCGCTGGACGCCATGGG
CCTGTCCGTTTCAGACGCCATACGCTTGCTCATGCTCAAGATTGCTGACGAACAGCGATTGCCGTTTGCGGTGCAAGTGC
CCAATGCCAAAACGGTCAAGGCCATGGAAGAGCTGGAAGCTGGCAAGGGCAAGCGTTTCAGTCACGAACAGGCGCTGTTT
GACGATCTGGGGTTGTAG