Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1610946..1611756 Replicon chromosome
Accession NZ_CP069961
Organism Klebsiella pneumoniae strain FDAARGOS_1317

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag I6K47_RS07855 Protein ID WP_004178461.1
Coordinates 1610946..1611479 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I6K47_RS07860 Protein ID WP_002887278.1
Coordinates 1611490..1611756 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K47_RS07850 1609777..1610898 + 1122 WP_020804439.1 cupin domain-containing protein -
I6K47_RS07855 1610946..1611479 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
I6K47_RS07860 1611490..1611756 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I6K47_RS07865 1611859..1613292 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
I6K47_RS07870 1613282..1613965 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
I6K47_RS07875 1614137..1615522 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
I6K47_RS07880 1615540..1615884 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T192322 WP_004178461.1 NZ_CP069961:c1611479-1610946 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T192322 NZ_CP092289:1042930-1043232 [Marinobacter sp. M1C]
ATGGCTAATTCCCTGGACAAACTTTTGGCGAGTGAGAAGCCGGAAGTCATAAAACGAGCGCAGGTTAAATCGGAGAATAT
TCTGTTAGAAATTCGTTTGAACGAAGTGCGCGCCTTATTGCAGAAAACACAAAAAGAGATGGCAAAGGCTATGGGCGTGA
CGCAGCCTACGATTGCAAGCATGGAGAAAATTGGAAAAGACTTGAAGCTGTCTTCCATTAAACGGTATGTGGAAAGCGTC
GGCGCGAAGGTTCGTCTGGATATTGAGCTTCCGGACGGAACCCACCATGCTATTCCGCTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT192322 WP_002887278.1 NZ_CP069961:c1611756-1611490 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT192322 NZ_CP092289:1043350-1043730 [Marinobacter sp. M1C]
ATGAGCGCACAAATTATCGAACGCGAAGGAAAGCCGGAGTATGCCGTATTGCCTTACCATGAGTACGTGCAGTTGATGGA
GCTGGCTGAAAGTGTGCGGGATGCGAGAGACGCAAGAGAAGCTATGCGTGAGCTCGACAGCGGCGAGGACGAAGCCATCC
CCGCCAACATCGTTGGCCGTTTGATCGCCGGAGACGACCACCCGCTGAAAATCTGGCGGGAGTATCGCAAGCTTACCCAA
GCAGCTTTGGGTAATGAAGCCGGGGTGGGCAAGTCCTATATCAGCCAGATTGAAACCGGCAGCAAAACTGGCTCAGCCAA
AGTGCTGAAGGCGCTGGCGGAAGCTCTGCAGGTGGATATGGATGATCTGTTGGTAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References