Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1011813..1012432 Replicon chromosome
Accession NZ_CP069961
Organism Klebsiella pneumoniae strain FDAARGOS_1317

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I6K47_RS04990 Protein ID WP_002892050.1
Coordinates 1012214..1012432 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I6K47_RS04985 Protein ID WP_002892066.1
Coordinates 1011813..1012187 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K47_RS04975 1006965..1008158 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K47_RS04980 1008181..1011327 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
I6K47_RS04985 1011813..1012187 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I6K47_RS04990 1012214..1012432 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
I6K47_RS04995 1012591..1013157 + 567 WP_002892030.1 maltose O-acetyltransferase -
I6K47_RS05000 1013129..1013269 - 141 WP_004147370.1 hypothetical protein -
I6K47_RS05005 1013290..1013760 + 471 WP_002892026.1 YlaC family protein -
I6K47_RS05010 1013735..1015186 - 1452 WP_004177237.1 PLP-dependent aminotransferase family protein -
I6K47_RS05015 1015287..1015985 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
I6K47_RS05020 1015982..1016122 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I6K47_RS05025 1016122..1016385 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192321 WP_002892050.1 NZ_CP069961:1012214-1012432 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T192321 NZ_CP092289:c1041231-1041049 [Marinobacter sp. M1C]
GTGACGAGCGATGAGGTTAAAAAGAAACTGGAAAAAGATGGCTGGGAGTTGGTGCGAGTGAATGGGAGCCATCATCACTT
TAAGCATTCCACAAAGCCGGGGCTGGTTACGCTGCCACACCCTAAGTCAGAACTGCCAAAGGGTACGTTGAACAATATCT
GGAAGCAGGCCGGTTGGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT192321 WP_002892066.1 NZ_CP069961:1011813-1012187 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT192321 NZ_CP092289:c1041008-1040577 [Marinobacter sp. M1C]
ATGCGTTTCCCAGTAGCACTTCATACAGATGATGGAAAGAACTACGGCGTTACTGTGCCCGATATTCCTGGCTGCTTCTC
TGCAGGCAGCAACGAAGACAAGGCTTTAGACAACGCACGTGAGGCAATTCTTGGTCACCTTGAGCTATTAGCAGAATTTG
GAGAAGACATTCCTACTGCCAACTCTATTGCCGAGCACCGTACAAACGCAGACTTTGAGGGTGCGGTGTGGGGCTTTATC
GAGATCGATGTAACACCACTCCTGGGCAAGGCAGAGAAGATCAATATCACCGTGCCCCGTTTGGTTTTGCGTCAGATTGA
CAGATATGTGAAGGAGCACCCGAACGAGGCGCGGTCACGCTCTGCGTTTCTATCTGAGGCAGCTTTGGAAAGGATGCGTA
AATCGCACGACAAGGCTAAAACCCTGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References