Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 19869..20416 Replicon plasmid unnamed1
Accession NZ_CP069958
Organism Escherichia coli strain FDAARGOS_1279

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag I6K09_RS24475 Protein ID WP_001384452.1
Coordinates 20138..20416 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag I6K09_RS24470 Protein ID WP_000079928.1
Coordinates 19869..20138 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K09_RS24445 (15514) 15514..16047 - 534 WP_001303319.1 transcription termination/antitermination NusG family protein -
I6K09_RS24450 (16489) 16489..16776 - 288 WP_000074849.1 conjugal transfer protein TraA -
I6K09_RS24455 (16694) 16694..16924 - 231 WP_228772074.1 ash family protein -
I6K09_RS24460 (17930) 17930..18961 + 1032 WP_000907875.1 plasmid replication initiator RepA -
I6K09_RS24465 (19656) 19656..19814 + 159 WP_000841008.1 hypothetical protein -
I6K09_RS24470 (19869) 19869..20138 + 270 WP_000079928.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
I6K09_RS24475 (20138) 20138..20416 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
I6K09_RS24480 (20456) 20456..21304 + 849 WP_001057996.1 3'-5' exonuclease -
I6K09_RS24485 (21541) 21541..21666 - 126 Protein_24 MerR family transcriptional regulator -
I6K09_RS24490 (21661) 21661..22842 + 1182 Protein_25 transposase -
I6K09_RS24495 (22907) 22907..23611 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
I6K09_RS24500 (23733) 23733..24638 + 906 WP_000219391.1 Mph(A) family macrolide 2'-phosphotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) - 1..94581 94581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T192309 WP_001384452.1 NZ_CP069958:20138-20416 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T192309 NZ_CP092288:c1921888-1921610 [Marinobacter sp. M2C]
ATGATTCGAAGCTTCAAACACAAGGGCTTGGCCAAATTCTTCAAGTCCGGCAGCATCGCAGGGATTCAGGCCGCTCACGC
CAAGAGGCTTCGGCTTATTCTCGGTAGATTAAATGCAGCCTCAGATGCCAAAGATATGGACCTGCCAGGCCTTCGGTTGC
ACGAACTCTCCGGTAATCGCGCCGGAATCTGGTCAGTGATGGTCAGTGGCAACTGGCGAGTGACGTTCCGATTTGAGGAC
GGAGATGCCGAGATCGTAAATTATGAAGATTACCATTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT192309 WP_000079928.1 NZ_CP069958:19869-20138 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT192309 NZ_CP092288:c1921599-1921312 [Marinobacter sp. M2C]
ATGTTGATGCATAACCCCCCCCACCCTGGTGAAGTACTGCGAGAGCTTTGTCTGGAGCCCCTGAACCTATCGGTCACGGC
GGCCGCAGAGGCATTGGGTGTTAGTCGTAAGACTTTGAGCGCCGTGCTGAACGGCAAGGCTGGTATTAGTCCCGAAATGG
CTATCCGGCTTTCTATAGCATTCGACACTTCGGCAGAGAGCTGGCTAAACCAGCAGTCCCAATATGAGCTCTGGCATGCT
GAACAACATCGCAAAGAGCTCAAGGTGAAGAAGCTTGTTGCGGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References