Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4252187..4252841 Replicon chromosome
Accession NZ_CP069957
Organism Escherichia coli strain FDAARGOS_1279

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K09_RS20390 Protein ID WP_000244781.1
Coordinates 4252434..4252841 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K09_RS20385 Protein ID WP_000354046.1
Coordinates 4252187..4252453 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K09_RS20365 (4248275) 4248275..4249708 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
I6K09_RS20370 (4249753) 4249753..4250064 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
I6K09_RS20375 (4250228) 4250228..4250887 + 660 WP_000250269.1 hemolysin III family protein -
I6K09_RS20380 (4250964) 4250964..4251944 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
I6K09_RS20385 (4252187) 4252187..4252453 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K09_RS20390 (4252434) 4252434..4252841 + 408 WP_000244781.1 protein YgfX Toxin
I6K09_RS20395 (4252881) 4252881..4253402 - 522 WP_001521233.1 flavodoxin FldB -
I6K09_RS20400 (4253514) 4253514..4254410 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6K09_RS20405 (4254435) 4254435..4255145 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K09_RS20410 (4255151) 4255151..4256884 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192306 WP_000244781.1 NZ_CP069957:4252434-4252841 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192306 NZ_CP092288:c1051677-1051423 [Marinobacter sp. M2C]
CTGGAACGATGCCGCAAATTCTTAGCCGCCAAAGCCCCGGAAGCCAGTAGGCGGGCAGGACAAACCATCGAACGGCACTT
CCTGTTTTTGGAAACAGCCACGGGTATTGGCCGGCCGTTCGCTGAAATGCCTGAATTGCGCGAATTAGTTATTGCCTTCG
GGAACTCCGGGTATGTGGCCCTGTACCACCATGAACCGGCCGACGATGCAGTATATGTTCTGGCCTTCCGGCACCAAAAA
GAAGCGGGCTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192306 WP_000354046.1 NZ_CP069957:4252187-4252453 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192306 NZ_CP092288:c1051976-1051704 [Marinobacter sp. M2C]
ATGGCAACATCTCTCAAAATCGACAACACACTAAAAAGCCGCGTTCATCACATCGCCAACCAACGCCGCCGCTCTCCGCA
CTGGATCATGCTGGAAGCCATACAGCAGTACGTTGAGCGCGAGGAAGCACGCGAGAGTTTCAAGCAGGAGGCCTTAGCGT
CTTGGTCAGCCTACAAGGAAACCGGCAGACACCTGACCGGCCAAGAAGCTCGCGCCTGGTTGAATACTTGGGGCAACGAT
GATGAGAAGGCGGCGCCAGAGTGCCACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References