Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2848434..2848931 Replicon chromosome
Accession NZ_CP069957
Organism Escherichia coli strain FDAARGOS_1279

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K09_RS13805 Protein ID WP_001173343.1
Coordinates 2848434..2848607 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K09_RS13810 Protein ID WP_000398619.1
Coordinates 2848635..2848931 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K09_RS13795 (2845104) 2845104..2846561 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
I6K09_RS13800 (2846798) 2846798..2848315 + 1518 WP_000003806.1 lysine--tRNA ligase -
I6K09_RS13805 (2848434) 2848434..2848607 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K09_RS13810 (2848635) 2848635..2848931 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K09_RS13815 (2849160) 2849160..2849432 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K09_RS13820 (2849444) 2849444..2849674 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K09_RS13825 (2849855) 2849855..2851486 + 1632 WP_001216477.1 sensor histidine kinase -
I6K09_RS13830 (2851483) 2851483..2852202 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2800549..2848931 48382


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192301 WP_001173343.1 NZ_CP069957:c2848607-2848434 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192301 NZ_CP092288:c543535-543125 [Marinobacter sp. M2C]
GTGACTACGTACATGCTCGACACCTGTATCTGCTCATACATCATGCGAGAGCATCCGGCGTCAGTGTTACAGCGATTGAC
CTACGAGGTTGAGCAGGGCGACCGAATTGTCATTTCAGCCATTACGTATGCAGAAATGCGATACGGACAAATCGGCAAGA
AAGCTTCGGCCAAGCACAAGGTGTTGATTGATGAATTCGTAGAGCGAGTTGACGCCGTTCTGTCATGGGATCAGAGGGCG
GTAGACGCGACAGTCACTGCGATGAGCCAGCTTCGCAAAGCAGGCACTCCCATTGGCCCTAACGACACGGCCATCGCGGG
ACATGCAATCGCCACGGGTTGCACTCTAGTAACGAACAATGTTCGTGAATTTTCTCGTGTTCCTAGTCTCGTTTACGAAG
ACTGGGTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT192301 WP_000398619.1 NZ_CP069957:c2848931-2848635 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192301 NZ_CP092288:c543762-543532 [Marinobacter sp. M2C]
ATGCGGACTGTTTCTATTTTCAAAAACGGCAAAAATCAGGCCGTGCGATTACCTGCTGACATGGCCTATACCGGCGTTGG
AGAGCTTGAAATTTCGCGAGACGGGGACGTAATCACTCTTCGCCCTGTCAGGGCATCTTGGGCTTCTTTTGCCGATCTCC
CAAAAGCGGACGCCGATTTCCTTAATGAGCGCCCCGTGGTCGCGAGTGATGAAAGCAGGTTTAATCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References