Detailed information of TA system TA192300

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2836651..2837486
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0A1A9A5
• Locus tag: I6K09_RS13760
• Protein ID: WP_001564063.1
• Coordinates: 2837109..2837486 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0A1AEL8
• Locus tag: I6K09_RS13755
• Protein ID: WP_038432125.1
• Coordinates: 2836651..2837019 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS13720 (2832310)	2832310..2832990	+	681	WP_001278649.1	WYL domain-containing protein	-
I6K09_RS13725 (2833141)	2833141..2833818	+	678	WP_001564058.1	hypothetical protein	-
I6K09_RS13730 (2833824)	2833824..2834057	+	234	WP_001278293.1	DUF905 family protein	-
I6K09_RS13735 (2834147)	2834147..2834965	+	819	WP_001175165.1	DUF932 domain-containing protein	-
I6K09_RS13740 (2835231)	2835231..2835710	+	480	WP_001564060.1	antirestriction protein	-
I6K09_RS13745 (2835726)	2835726..2836202	+	477	WP_021553055.1	RadC family protein	-
I6K09_RS13750 (2836267)	2836267..2836488	+	222	WP_000692345.1	DUF987 domain-containing protein	-
I6K09_RS13755 (2836651)	2836651..2837019	+	369	WP_038432125.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K09_RS13760 (2837109)	2837109..2837486	+	378	WP_001564063.1	TA system toxin CbtA family protein	Toxin
I6K09_RS13765 (2837483)	2837483..2837632	+	150	Protein_2723	DUF5983 family protein	-
I6K09_RS13770 (2837711)	2837711..2837905	+	195	WP_024181941.1	DUF957 domain-containing protein	-
I6K09_RS13775 (2837990)	2837990..2838832	+	843	Protein_2725	DUF4942 domain-containing protein	-
I6K09_RS13780 (2839581)	2839581..2841119	+	1539	WP_001187196.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2800549..2848931	48382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14094.09 Da
• Isoelectric Point: 7.8045

>T192300 WP_001564063.1 NZ_CP069957:2837109-2837486 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T192300 NZ_CP092286:9839-10027 [Marinobacter sp. M3C]
ATGAAAAGCGCAGACATTATCAAACGATTGAAGGCGGAAGGCTGGGTGAAAGTGGGCGGCAAAGGTGACCACGAAAAGTT
CAAGCACCCGGAAAAGCCCGGACACGTAGTGGTGCCGCACCCGCGAAAGGATATGCCGCTGGGTACTCTGCGCAGCATCT
ACAGGCAAGCAGGATGGGATTGGAGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13569.31 Da
• Isoelectric Point: 7.0369

>AT192300 WP_038432125.1 NZ_CP069957:2836651-2837019 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT192300 NZ_CP092286:10030-10419 [Marinobacter sp. M3C]
ATGTTATTCCCCGTTTACGTACACACAGGCGACGACACACACGCCCATGGCGCCGAAGTGCCAGACTTCCCCGGCTGCTT
TTCAGCGGCGGACAACTGGGACGCGCTGGCGGGTAACATTCAAGAAGCGGTAGAGTTGCACTGCGAAGATGAGGATATGG
CTGTGCCAGCGCCCACCTCGCTAGGAAAACTGATGGGCAGCCCAGATTACCAAGGCGGCATTTGGATGATGGTGGATATT
GACACTGGCAAGCTTAGAACAAAGTCTGTGCGGCTGAACGTAAGCTTGCCAGAAGGCTTGCTGCGACGTATTGATGACGA
GGCCAAGATCCGCCACATGAGCCGGTCTGCCTTTTTGGCAATGTCAGCACAGCGGGAACTTGGAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0A1A9A5

Antitoxin

• AlphaFold DB: A0A0A1AEL8