Detailed information of TA system TA192297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2557014..2557828
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: I6K09_RS12305
• Protein ID: WP_001054376.1
• Coordinates: 2557014..2557271 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: I6K09_RS12310
• Protein ID: WP_001309181.1
• Coordinates: 2557283..2557828 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS25930 (2552044)	2552044..2552249	+	206	Protein_2423	HNH endonuclease	-
I6K09_RS12280 (2552517)	2552517..2553646	-	1130	Protein_2424	helicase YjhR	-
I6K09_RS12285 (2553733)	2553733..2554158	+	426	WP_000422741.1	transposase	-
I6K09_RS12290 (2554155)	2554155..2554505	+	351	WP_000624718.1	IS66 family insertion sequence element accessory protein TnpB	-
I6K09_RS12295 (2554536)	2554536..2556128	+	1593	WP_048237919.1	IS66 family transposase	-
I6K09_RS25935 (2556392)	2556392..2556523	+	132	WP_001309182.1	hypothetical protein	-
I6K09_RS12305 (2557014)	2557014..2557271	+	258	WP_001054376.1	YjhX family toxin	Toxin
I6K09_RS12310 (2557283)	2557283..2557828	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
I6K09_RS12315 (2557884)	2557884..2558630	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
I6K09_RS12320 (2558799)	2558799..2559017	+	219	Protein_2432	hypothetical protein	-
I6K09_RS12325 (2559055)	2559055..2559171	+	117	Protein_2433	VOC family protein	-
I6K09_RS12330 (2559416)	2559416..2560537	+	1122	WP_000010829.1	M42 family metallopeptidase	-
I6K09_RS12335 (2560534)	2560534..2560812	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
I6K09_RS12340 (2560824)	2560824..2562137	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T192297 WP_001054376.1 NZ_CP069957:2557014-2557271 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T192297 NZ_CP092284:2641320-2641571 [Marinobacter sp. M3C]
ATGGATGCCATCAGCTACACAGCCGCTAGAACTAACCTAGCAAAAACCATGGAGCAGGTCTGTGAAGACCATTCTCCTGT
GATCATCACCCGGAGCAAGTCACAATCCGTGGTGATGATTTCTCTTGAGGACTATGAGGCGCTGCAAGAGACTGCATACT
TGCTGCGCGCACCAAAAAATGCCCGTCGTTTGCTGGAATCCGTTGCCGAGCTCGAGCAAGGCGGTGGTCAAGAAAAGGAA
CTTCTTGAATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT192297 WP_001309181.1 NZ_CP069957:2557283-2557828 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT192297 NZ_CP092284:2641568-2641822 [Marinobacter sp. M3C]
ATGAAACTTATCTTCTCCGAGAACGCCTGGGAAGACTATCTGTATTGGCAGAAAACTGACAAAAAAGCCCTTAACCGAAT
CAACAAGCTGATCAAGGAGACTAAGCGAGAACCGTTCGAAGGTGTCGGTAAGCCGGAGCCCCTCAAACACAACCTCGCTG
GTTACTGGTCCCGCCGAATTAACGAAGAGCACCGCATCGTTTACAAAGTCACGGATGACGCTTTACTCATAGCCCAACTC
CGTTACCACTACTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8