Detailed information of TA system TA192295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2144284..2145973
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: I6K09_RS10380
• Protein ID: WP_001521912.1
• Coordinates: 2144284..2145204 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: I6K09_RS10385
• Protein ID: WP_001521911.1
• Coordinates: 2145191..2145973 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS10365 (2140374)	2140374..2141501	-	1128	WP_001521913.1	MFS transporter	-
I6K09_RS10370 (2141643)	2141643..2142701	-	1059	WP_122452191.1	alpha/beta hydrolase	-
I6K09_RS10375 (2142966)	2142966..2143850	+	885	WP_001119037.1	LysR family transcriptional regulator	-
I6K09_RS10380 (2144284)	2144284..2145204	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I6K09_RS10385 (2145191)	2145191..2145973	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
I6K09_RS10390 (2146289)	2146289..2149498	+	3210	WP_048237838.1	RNA-directed DNA polymerase	-
I6K09_RS10400 (2149845)	2149845..2150315	+	471	WP_000201185.1	hypothetical protein	-
I6K09_RS10405 (2150312)	2150312..2150414	+	103	Protein_2057	helix-turn-helix domain-containing protein	-
I6K09_RS10410 (2150506)	2150506..2150664	+	159	Protein_2058	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T192295 WP_001521912.1 NZ_CP069957:c2145204-2144284 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T192295 NZ_CP092284:c2613008-2612745 [Marinobacter sp. M3C]
GTGACATGGAAGTTGGTTTACACCAAACAGGCCCAAAAAGATGCAAAAAAGTTAGCTGCCAGCAGCCTCAAGCCAAAAGC
TCAGGGATTGTTGGCGCTGATTGCGGAAGATCCGTATCGGAGGCCGCCGCCGTTCGAAAAGCTCATTGGTGATCTTGCAG
GAGCATACTCTCGCCGCATCAATATCCAGCATCGTGTTGTTTACGAAGTGCTCGAAGATGAACGAGTGGTAAAAGTTCTC
CGGCTCTGGAGTCACTACGAATAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT192295 WP_001521911.1 NZ_CP069957:c2145973-2145191 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT192295 NZ_CP092284:c2613247-2613005 [Marinobacter sp. M3C]
ATGACCGGAATTACGGCAACTGAGGCGCGCAGCAACCTTTACAGGTTGATTGACGAAACGGCCGAGTCCCATCAACCAAT
CGTTATTATGGGTAAACGGAACAGGGCTGTTCTCGTTTCCGAGGAAGATTGGTCTGCGATTCAGGAAACGCTTTACCTGT
TATCTGTACCAGGTATGCGCGAGTCTATTCGAGAGGGAATGGAGACCCCTATGGATCAATGCGATGAGGAGCTGAACTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A829L165