192294

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1946651..1947269 Replicon chromosome
Accession NZ_CP069957
Organism Escherichia coli strain FDAARGOS_1279

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K09_RS09450 Protein ID WP_001291435.1
Coordinates 1947051..1947269 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K09_RS09445 Protein ID WP_000344800.1
Coordinates 1946651..1947025 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K09_RS09435 (1941740) 1941740..1942933 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K09_RS09440 (1942956) 1942956..1946105 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K09_RS09445 (1946651) 1946651..1947025 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K09_RS09450 (1947051) 1947051..1947269 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K09_RS09455 (1947441) 1947441..1947992 + 552 WP_001522034.1 maltose O-acetyltransferase -
I6K09_RS09460 (1948108) 1948108..1948578 + 471 WP_001490888.1 YlaC family protein -
I6K09_RS09465 (1948742) 1948742..1950292 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K09_RS09470 (1950334) 1950334..1950687 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K09_RS09480 (1951066) 1951066..1951377 + 312 WP_000409911.1 MGMT family protein -
I6K09_RS09485 (1951408) 1951408..1951980 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192294 WP_001291435.1 NZ_CP069957:1947051-1947269 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192294 NZ_CP092284:1657910-1658092 [Marinobacter sp. M3C]
GTGACGAGCGATGAGGTTAAAAAGAAACTGGAAAAAGATGGCTGGGAGTTGGTGCGAGTGAATGGGAGCCATCATCACTT
TAAGCATTCCACAAAGCCGGGGCTGGTTACGCTGCCACACCCTAAGTCAGAACTGCCAAAGGGTACGTTGAACAATATCT
GGAAGCAGGCCGGTTGGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192294 WP_000344800.1 NZ_CP069957:1946651-1947025 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192294 NZ_CP092284:1658133-1658564 [Marinobacter sp. M3C]
ATGCGTTTCCCAGTAGCACTTCATACAGATGATGGAAAGAACTACGGCGTTACTGTGCCCGATATTCCTGGCTGCTTCTC
TGCAGGCAGCAACGAAGACAAGGCGTTAGACAACGCACGTGAGGCAATTCTTGGTCACCTTGAGCTATTAGCAGAATTTG
GAGAAGACATTCCTACTGCCAACTCTATTGCCGAGCACCGTACAAACGCAGACTTTAAGGGTGCGGTGTGGGGCTTTATC
GAGATCGATGTAACACCATTCCTGGGCAAGGCGGAGAAGATCAATATCACCGTGCCCCGTTTGGTTTTGCGTCAGATTGA
CAGATATGTGAAGGAGCACCCGAACGAGGCGCGGTCACGCTCTGCGTTTCTATCTGAGGCAGCTTTGGAAAGGATGCGTA
AATCGCACGACAAGGCTAAGACCCTGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References