Detailed information of TA system TA192293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1524253..1524958
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K09_RS07595
• Protein ID: WP_000539521.1
• Coordinates: 1524253..1524639 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K09_RS07600
• Protein ID: WP_001280945.1
• Coordinates: 1524629..1524958 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS07575 (1520257)	1520257..1520883	+	627	WP_020233670.1	glutathione S-transferase GstB	-
I6K09_RS07580 (1520880)	1520880..1521995	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
I6K09_RS07585 (1522106)	1522106..1522489	-	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K09_RS07590 (1522702)	1522702..1524027	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K09_RS07595 (1524253)	1524253..1524639	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K09_RS07600 (1524629)	1524629..1524958	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K09_RS07605 (1525028)	1525028..1526356	-	1329	WP_000086873.1	GGDEF domain-containing protein	-
I6K09_RS07610 (1526364)	1526364..1528712	-	2349	WP_021523051.1	EAL domain-containing protein	-
I6K09_RS07615 (1528890)	1528890..1529801	-	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1515721..1524958	9237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192293 WP_000539521.1 NZ_CP069957:1524253-1524639 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192293 NZ_CP092284:c1656211-1655909 [Marinobacter sp. M3C]
ATGGCTAATTCCCTGGACAAACTTTTGGCGAGTGAGAAGCCGGAAGTCATAAAACGAGCGCAGGTTAAATCGGAGAATAT
TCTGTTAGAAATTCGTTTGAACGAAGTGCGCGCCTTATTGCAGAAAACACAAAAAGAGATGGCAAAGGCTATGGGCGTGA
CGCAGCCTACGATTGCAAGCATGGAGAAAATTGGAAAAGACTTGAAGCTGTCTTCCATTAAACGGTATGTGGAAAGCGTC
GGCGCGAAGGTTCGTCTGGATATTGAGCTTCCGGACGGAACCCACCATGCTATTCCGCTGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192293 WP_001280945.1 NZ_CP069957:1524629-1524958 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192293 NZ_CP092284:c1655791-1655411 [Marinobacter sp. M3C]
ATGAGCGCACAAATTATCGAACGCGAAGGAAAGCCGGAGTATGCCGTATTGCCTTACCATGAGTACGTGCAGTTGATGGA
GCTGGCTGAAAGTGTGCGGGATGCGAGAGACGCAAGAGAAGCTATGCGTGAGCTCGACAGCGGCGAGGACGAAGCCATCC
CCGCCAACATCGTTGGCCGTTTGATCGCCGGAGACGACCACCCGCTGAAAATCTGGCGGGAGTATCGCAAGCTTACCCAA
GCAGCTTTGGGTAATGAAGCCGGGGTGGGCAAGTCCTATATCAGCCAGATTGAAACCGGCAGCAAAACTGGCTCAGCCAA
AGTGCTGAAGGCGCTGGCGGAAGCTCTGCAGGTGGATATGGATGATCTGTTGGTAGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6