Detailed information of TA system TA192292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1230074..1230858
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K09_RS06160
• Protein ID: WP_000613626.1
• Coordinates: 1230364..1230858 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K09_RS06155
• Protein ID: WP_001110447.1
• Coordinates: 1230074..1230367 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS06145 (1225224)	1225224..1226183	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
I6K09_RS06150 (1226756)	1226756..1229941	+	3186	WP_001522867.1	ribonuclease E	-
I6K09_RS06155 (1230074)	1230074..1230367	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K09_RS06160 (1230364)	1230364..1230858	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K09_RS06165 (1230953)	1230953..1231906	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K09_RS06170 (1231918)	1231918..1233561	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
I6K09_RS06175 (1233627)	1233627..1234568	-	942	WP_048237783.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K09_RS06180 (1234568)	1234568..1235665	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192292 WP_000613626.1 NZ_CP069957:1230364-1230858 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192292 NZ_CP092284:1653810-1654082 [Marinobacter sp. M3C]
GTGCTAACGCCAGTGCAATCGACCCAGTTCAAAAAAGACGTGAAGAAAGCCCGCAAGCGGGGCAAAGACTTGGACAAACT
AAAGATGTCGCTCAGTTTGCTGATTCGCCAGGCACCGTTACCCGAAGCTTATCAAGATCATCCTTTACGGGGGAACTGGA
AGGGCTATCGAGACGCGCATATTGAGCCGGACTGGTTGCTTTTGTACCGAGTGACCGACAAGGAATTGCAGTTAGCCCGA
ACCGGAACGCACGCGGATTTGTTTCAGGAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192292 WP_001110447.1 NZ_CP069957:1230074-1230367 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192292 NZ_CP092284:1653553-1653810 [Marinobacter sp. M3C]
ATGAATACCGATTCTGTAGTTCGTGCCCGCATCGACAGTGACACCAAAGCCCGCGCCACCGCCGCGCTGGACGCCATGGG
CCTGTCCGTTTCAGACGCCATACGCTTGCTCATGCTCAAGATTGCTGACGAACAGCGATTGCCGTTTGCGGTGCAAGTGC
CCAATGCCAAAACGGTCAAGGCCATGGAAGAGCTGGAAGCTGGCAAGGGCAAGCGTTTCAGTCACGAACAGGCGCTGTTT
GACGATCTGGGGTTGTAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6