Detailed information of TA system TA192291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 789699..790337
• Replicon: chromosome
• Accession: NZ_CP069957
• Organism: Escherichia coli strain FDAARGOS_1279

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: I6K09_RS03890
• Protein ID: WP_001306887.1
• Coordinates: 790161..790337 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K09_RS03885
• Protein ID: WP_001270286.1
• Coordinates: 789699..790115 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K09_RS03865 (784851)	784851..785792	-	942	WP_001251315.1	ABC transporter permease	-
I6K09_RS03870 (785793)	785793..786806	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
I6K09_RS03875 (786824)	786824..787969	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
I6K09_RS03880 (788214)	788214..789620	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
I6K09_RS03885 (789699)	789699..790115	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K09_RS03890 (790161)	790161..790337	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K09_RS03895 (790559)	790559..790789	+	231	WP_000494241.1	YncJ family protein	-
I6K09_RS03900 (790881)	790881..792842	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K09_RS03905 (792915)	792915..793451	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
I6K09_RS03910 (793543)	793543..794715	+	1173	WP_001523225.1	BenE family transporter YdcO	-
I6K09_RS03915 (794823)	794823..795281	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	794823..795281	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T192291 WP_001306887.1 NZ_CP069957:c790337-790161 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T192291 NZ_CP092284:1649820-1650110 [Marinobacter sp. M3C]
ATGAGCTCTAAGTACGCTCTAGAATTCAAACCCTCTGCCCTTAAAGAATGGAAGAAGCTCGCACCGGCGCTGCAGGCTCA
GTTTAAGAAAAAGCTCAAACAGCGGCTCGACGATCCTCACGTGCCGCCAGACGCCCTATCCGATCTCAAGAACTGCTACA
AAATCAAACTTCGCAGCGCAGGTTACCGATTGGTTTATGAGGTCGAAGACGAACGTATCGTTGTAACGGTGGTCGCCGTG
GGCAAGCGAGAGCGTGGTGCGGTGTACAGTCGGGCTCACCGACGAATCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT192291 WP_001270286.1 NZ_CP069957:c790115-789699 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192291 NZ_CP092284:1649582-1649830 [Marinobacter sp. M3C]
ATGACTCAACCCATTTATACCGAAATTGCGGCTAGTATCTCGGAGTTAAAGAAAAACCCAATGGCAGCTGTTAGTGCCGG
CGAGGGGTTTCCCATCGTTGTTTTGAACCGAAATAAACCAGCATTCTACTGCGTGCCAGCGGATGTTTACGAGGCTATGC
TGGATCGCCTGGACGATCAAGAATTGGCTCGCCTGGTGGAGTCGCGCCGATCAGAACGCAGCATCCCGGTGTCCCTGAAT
GAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76