Detailed information of TA system TA192268

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4171202..4172044
• Replicon: chromosome
• Accession: NZ_CP069946
• Organism: Klebsiella pneumoniae strain FDAARGOS_1325

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6K55_RS20440
• Protein ID: WP_085393668.1
• Coordinates: 4171667..4172044 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K55_RS20435
• Protein ID: WP_077256055.1
• Coordinates: 4171202..4171615 (+)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K55_RS20390	4166689..4167897	+	1209	WP_085393675.1	IS256 family transposase	-
I6K55_RS20395	4168057..4168359	+	303	WP_085393674.1	hypothetical protein	-
I6K55_RS20400	4168417..4168728	+	312	WP_085393673.1	hypothetical protein	-
I6K55_RS20405	4168780..4169250	+	471	WP_085393672.1	hypothetical protein	-
I6K55_RS20410	4169324..4169545	+	222	WP_085393671.1	hypothetical protein	-
I6K55_RS20415	4169601..4169790	+	190	Protein_3991	DUF905 family protein	-
I6K55_RS20420	4170030..4170500	+	471	WP_085393670.1	antirestriction protein	-
I6K55_RS20425	4170512..4170991	+	480	WP_085393669.1	DNA repair protein RadC	-
I6K55_RS20430	4171012..4171233	+	222	WP_040246532.1	DUF987 domain-containing protein	-
I6K55_RS20435	4171202..4171615	+	414	WP_077256055.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K55_RS20440	4171667..4172044	+	378	WP_085393668.1	TA system toxin CbtA family protein	Toxin
I6K55_RS20445	4172094..4172525	+	432	WP_085393667.1	hypothetical protein	-
I6K55_RS20450	4172555..4172758	+	204	WP_085393666.1	DUF957 domain-containing protein	-
I6K55_RS20455	4172840..4173685	+	846	WP_085393665.1	DUF4942 domain-containing protein	-
I6K55_RS20460	4173829..4174626	+	798	WP_085393664.1	helix-turn-helix transcriptional regulator	-
I6K55_RS20465	4174942..4176053	+	1112	Protein_4001	IS3 family transposase	-
I6K55_RS20470	4176070..4176258	+	189	Protein_4002	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4149662..4184855	35193
	-	flank	IS/Tn	-	-	4166689..4167897	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13960.80 Da
• Isoelectric Point: 5.0715

>T192268 WP_085393668.1 NZ_CP069946:4171667-4172044 [Klebsiella pneumoniae]
MQTQPLSSTQEASPRPSPVDIWQQLLSYLLDRHYGLTLNDTPLGHDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRPGF
SAETQLPLLNSIDILRARKACGLMTRNDYRTVTDITTGKYCGVQP

Nucleotide

• Length: 378 bp

>T192268 NZ_CP092283:c1042912-1042730 [Marinobacter sp. M4C]
GTGACGAGCGATGAGGTTAAAAAGAAACTGGAAAAAGATGGCTGGGAGTTGGTGCGAGTGAATGGGAGCCATCATCACTT
TAAGCATTCCACAAAGCCGGGGCTGGTTACGCTGCCACACCCTAAGTCAGAACTGCCAAAGGGTACGTTGAACAATATCT
GGAAGCAGGCCGGTTGGAAATAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14964.07 Da
• Isoelectric Point: 6.8319

>AT192268 WP_077256055.1 NZ_CP069946:4171202-4171615 [Klebsiella pneumoniae]
MSSCAVSRSINVNLRKAKLSNEIPAVNHDIAEPWWGLKPTVTPCFGARLVQEGNQVHYLADRASITGQFSEADLRHLDQA
FPVLLKQLELMLVSGELNPHHQHGVTLYAKGLTCEADSLGSHGYIYIAIYPTPAATA

Nucleotide

• Length: 414 bp

>AT192268 NZ_CP092283:c1042689-1042258 [Marinobacter sp. M4C]
ATGCGTTTCCCAGTAGCACTTCATACAGATGATGGAAAGAACTACGGCGTTACTGTGCCCGATATTCCTGGCTGCTTCTC
TGCAGGCAGCAACGAAGACAAGGCTTTAGACAACGCACGTGAGGCAATTCTTGGTCACCTTGAGCTATTAGCAGAATTTG
GAGAAGACATTCCTACTGCCAACTCTATTGCCGAGCACCGTACAAACGCAGACTTTGAGGGTGCGGTGTGGGGCTTTATC
GAGATCGATGTAACACCACTCCTGGGCAAGGCAGAGAAGATCAATATCACCGTGCCCCGTTTGGTTTTGCGTCAGATTGA
CAGATATGTGAAGGAGCACCCGAACGAGGCGCGGTCACGCTCTGCGTTTCTATCTGAGGCAGCTTTGGAAAGGATGCGTA
AATCGCACGACAAGGCTAAAACCCTGGCATAA