Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2040614..2041257 Replicon chromosome
Accession NZ_CP069946
Organism Klebsiella pneumoniae strain FDAARGOS_1325

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag I6K55_RS10085 Protein ID WP_032433387.1
Coordinates 2040614..2041030 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag I6K55_RS10090 Protein ID WP_001261276.1
Coordinates 2041027..2041257 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K55_RS10055 2035783..2035986 - 204 WP_032433376.1 hemolysin expression modulator Hha -
I6K55_RS10060 2036046..2036537 - 492 WP_032433378.1 hypothetical protein -
I6K55_RS10065 2036568..2037086 - 519 WP_045326794.1 hypothetical protein -
I6K55_RS10070 2037145..2037393 - 249 WP_032433382.1 hypothetical protein -
I6K55_RS10075 2037712..2039283 + 1572 WP_032433383.1 ATP-binding protein -
I6K55_RS10080 2039478..2040458 - 981 WP_032433385.1 hypothetical protein -
I6K55_RS10085 2040614..2041030 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K55_RS10090 2041027..2041257 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6K55_RS10095 2041562..2042377 + 816 WP_032433388.1 hypothetical protein -
I6K55_RS10100 2042905..2045145 - 2241 WP_045326788.1 NTPase -
I6K55_RS10105 2045148..2046235 - 1088 Protein_1996 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2012887..2056621 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T192261 WP_032433387.1 NZ_CP069946:c2041030-2040614 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T192261 NZ_CP092258:c4547469-4547095 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAGCGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT192261 WP_001261276.1 NZ_CP069946:c2041257-2041027 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT192261 NZ_CP092258:c4547711-4547469 [Salmonella enterica subsp. enterica serovar Indiana]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References