Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2023830..2024566 Replicon chromosome
Accession NZ_CP069946
Organism Klebsiella pneumoniae strain FDAARGOS_1325

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag I6K55_RS09990 Protein ID WP_032433360.1
Coordinates 2023830..2024312 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K55_RS09995 Protein ID WP_003026799.1
Coordinates 2024300..2024566 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K55_RS09970 2020379..2021119 + 741 WP_050597964.1 molecular chaperone -
I6K55_RS09975 2021123..2021701 + 579 WP_032432061.1 type 1 fimbrial protein -
I6K55_RS09980 2021713..2022657 + 945 WP_077254249.1 fimbrial protein -
I6K55_RS09985 2022870..2023469 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
I6K55_RS09990 2023830..2024312 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
I6K55_RS09995 2024300..2024566 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K55_RS10000 2024879..2025442 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
I6K55_RS10005 2025452..2026084 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
I6K55_RS10010 2026102..2027106 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
I6K55_RS10015 2027090..2027752 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
I6K55_RS10020 2027781..2028920 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2012887..2056621 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T192260 WP_032433360.1 NZ_CP069946:c2024312-2023830 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T192260 NZ_CP092258:c3858176-3857763 [Salmonella enterica subsp. enterica serovar Indiana]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCATCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT192260 WP_003026799.1 NZ_CP069946:c2024566-2024300 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192260 NZ_CP092258:c3858423-3858157 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References