Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3901061..3901715 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6L03_RS18445 Protein ID WP_000244781.1
Coordinates 3901061..3901468 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6L03_RS18450 Protein ID WP_000354046.1
Coordinates 3901449..3901715 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS18425 (3897018) 3897018..3898751 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
I6L03_RS18430 (3898757) 3898757..3899467 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6L03_RS18435 (3899492) 3899492..3900388 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6L03_RS18440 (3900500) 3900500..3901021 + 522 WP_001055874.1 flavodoxin FldB -
I6L03_RS18445 (3901061) 3901061..3901468 - 408 WP_000244781.1 protein YgfX Toxin
I6L03_RS18450 (3901449) 3901449..3901715 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6L03_RS18455 (3901958) 3901958..3902938 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
I6L03_RS18460 (3903015) 3903015..3903674 - 660 WP_000250274.1 hemolysin III family protein -
I6L03_RS18465 (3903838) 3903838..3904149 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6L03_RS18470 (3904194) 3904194..3905627 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192245 WP_000244781.1 NZ_CP069945:c3901468-3901061 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192245 NZ_CP092252:2762699-2762806 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192245 WP_000354046.1 NZ_CP069945:c3901715-3901449 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192245 NZ_CP092252:c2762646-2762585 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References