Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3698458..3699041 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag I6L03_RS17550 Protein ID WP_000254750.1
Coordinates 3698458..3698793 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag I6L03_RS17555 Protein ID WP_000581937.1
Coordinates 3698793..3699041 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS17535 (3694344) 3694344..3695642 - 1299 WP_000036723.1 phosphopyruvate hydratase -
I6L03_RS17540 (3695730) 3695730..3697367 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
I6L03_RS17545 (3697595) 3697595..3698386 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
I6L03_RS17550 (3698458) 3698458..3698793 - 336 WP_000254750.1 endoribonuclease MazF Toxin
I6L03_RS17555 (3698793) 3698793..3699041 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
I6L03_RS17560 (3699119) 3699119..3701353 - 2235 WP_000226815.1 GTP pyrophosphokinase -
I6L03_RS17565 (3701401) 3701401..3702702 - 1302 WP_000046819.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T192243 WP_000254750.1 NZ_CP069945:c3698793-3698458 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T192243 NZ_CP092252:c2504230-2504054 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT192243 WP_000581937.1 NZ_CP069945:c3699041-3698793 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT192243 NZ_CP092252:c2504008-2503592 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References