Detailed information of TA system TA192230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1937922..1938706
• Replicon: chromosome
• Accession: NZ_CP069945
• Organism: Escherichia coli strain FDAARGOS_1373

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6L03_RS09010
• Protein ID: WP_000613626.1
• Coordinates: 1937922..1938416 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6L03_RS09015
• Protein ID: WP_001110447.1
• Coordinates: 1938413..1938706 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L03_RS08990 (1933114)	1933114..1934211	+	1098	WP_001764422.1	flagellar basal body P-ring protein FlgI	-
I6L03_RS08995 (1934211)	1934211..1935152	+	942	WP_001764423.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6L03_RS09000 (1935218)	1935218..1936861	+	1644	WP_001764424.1	flagellar hook-associated protein FlgK	-
I6L03_RS09005 (1936874)	1936874..1937827	+	954	WP_001212764.1	flagellar hook-associated protein FlgL	-
I6L03_RS09010 (1937922)	1937922..1938416	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6L03_RS09015 (1938413)	1938413..1938706	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6L03_RS09020 (1938839)	1938839..1942024	-	3186	WP_001764425.1	ribonuclease E	-
I6L03_RS09025 (1942597)	1942597..1943556	+	960	WP_000846326.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192230 WP_000613626.1 NZ_CP069945:c1938416-1937922 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192230 NZ_CP092206:2616058-2616456 [Leptospira weilii]
ATGTATCTTTTGGGTAGTAATATTTGTATTTTCTTAATTAAAAAAAAGAATACAATTCTTTTGGAAAACCTTAAGAAAAA
ATTAAATAAAGGTATATTTATTTCCTCTTTAACTATTGCGGAACTTGAATTTGGAATTCAGAAAAGTGAATTTAAGGAAA
GAAACAAAGTTGCCCTGATTGAATTTTTAACAATTTTCGATATACTTTCTTTTTCTGATAAAGATGCAGAGTCCTACGGT
TTAATTAGAGCTAATTTGGAGAGAAAAGGTAATATAATTGGTTCAATAGATATGCTTCTTGCTGCTCAAGCAATTGCAAA
TAATTATATTTTTGTTACTAATAATACAAAAGAATTTAAACGGATTAAGGATTTAAAAATTGAAGATTGGACTCGATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192230 WP_001110447.1 NZ_CP069945:c1938706-1938413 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192230 NZ_CP092206:2615828-2616058 [Leptospira weilii]
ATGCAGACAGCAAAATTATTTATAAATGGAAGAAGTCAAGCGGTTAGATTGCCAAAAGAATTTCAATTTACTGGAGATGA
TGTCCTTATTCAAAAGGTCGGAGAGGCCGTAATATTGGTTCCGAAGAACAAGGCTTGGAATGTATTTTTAGAAGGATTAA
ATGGGTTTTCAAATGATTTTCTCGGAAAAGGGAGGGAACAACCGAAATTCGATAAAAGAGATAAGTTTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6