Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1335141..1335759 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6L03_RS06155 Protein ID WP_001291435.1
Coordinates 1335141..1335359 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6L03_RS06160 Protein ID WP_000344800.1
Coordinates 1335385..1335759 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS06120 (1330428) 1330428..1331000 + 573 WP_000779831.1 YbaY family lipoprotein -
I6L03_RS06125 (1331031) 1331031..1331342 - 312 WP_000409908.1 MGMT family protein -
I6L03_RS06135 (1331721) 1331721..1332074 + 354 WP_000878138.1 DUF1428 family protein -
I6L03_RS06140 (1332116) 1332116..1333666 - 1551 WP_001764308.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6L03_RS06145 (1333830) 1333830..1334300 - 471 WP_000136192.1 YlaC family protein -
I6L03_RS06150 (1334416) 1334416..1334967 - 552 WP_000102539.1 maltose O-acetyltransferase -
I6L03_RS06155 (1335141) 1335141..1335359 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6L03_RS06160 (1335385) 1335385..1335759 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6L03_RS06165 (1336304) 1336304..1339453 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6L03_RS06170 (1339476) 1339476..1340669 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192228 WP_001291435.1 NZ_CP069945:c1335359-1335141 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192228 NZ_CP092204:22003-22344 [Leptospira weilii]
TTGGTAAAGAGTAGGAAATATACTCCTGAAAAAGGAGATATAGTTTGGCTGAACTTTACCCCGCAAGCAGGTCATGAACA
AAAAGGTCGTAGACCAGCTTTAGTTTTGTCACCTAAAGAATATAATTCGAAAACTGGATTAGCAATTTTCTGTCCGATTA
CTAGTAAGATTAAAGGTTATCCATTCGAGGTAACAATTAAATTGAAGAAAATTGATGGTGTAATTCTTTCTGATCAAGTG
AAAAACTTAGACTGGACTATTCGTGAAGCTGAATTTATAGAATCAATAAATAAAGTTTCTTTGAAAGAAGTTTTAGATAA
TATTAAGTTATTAGTATTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192228 WP_000344800.1 NZ_CP069945:c1335759-1335385 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192228 NZ_CP092204:21773-22009 [Leptospira weilii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGACGGAAGCCACGTTGAATTACAATATGAAGGGGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTAGAAGAAA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCAACTGGTAACTCAATAGGTAAAGAAGCTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References