Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 836702..837252 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6L03_RS03890 Protein ID WP_001160966.1
Coordinates 836938..837252 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6L03_RS03885 Protein ID WP_000125566.1
Coordinates 836702..836935 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS03860 (831775) 831775..832062 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6L03_RS03865 (832121) 832121..833452 + 1332 WP_001183207.1 MFS transporter -
I6L03_RS03870 (833560) 833560..834090 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6L03_RS03875 (834083) 834083..835945 + 1863 WP_001765385.1 glutathione-regulated potassium-efflux system protein KefC -
I6L03_RS03880 (836137) 836137..836616 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6L03_RS03885 (836702) 836702..836935 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6L03_RS03890 (836938) 836938..837252 + 315 WP_001160966.1 CcdB family protein Toxin
I6L03_RS03895 (837249) 837249..838097 - 849 WP_001765384.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6L03_RS03900 (838104) 838104..838481 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6L03_RS03905 (838484) 838484..839305 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6L03_RS03910 (839302) 839302..840291 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6L03_RS03915 (840291) 840291..841577 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T192226 WP_001160966.1 NZ_CP069945:836938-837252 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T192226 NZ_CP092203:c3456737-3456336 [Leptospira weilii]
ATGACTCAATACTTGTTGGATACGAATATCTGTATCTATATAATTAATCAAAAGCCGGAGTCTGTTTATAAGAAATTTAA
AAAGATTAAATTAGAGAATATTTTCATTTCATCAATTACGGAATTTGAATTAAAATACGGTGTTCAGAAGAGTCTTCATT
TTGAAAGAAATCTGAAAGTCTTAGAAGAATTTTTAAGTTATTTGAATATTCTTCCGTTTGTTAGTGAAGATACGAATAAA
GCTGCAAAAGTTCGAGTAGAATTAGAAAGAGTAGGGAAACCAATTGGTCCTTTCGATCTTCTAATCGCTTCTCAGGCATT
ATCGAATAAACTTACGCTTGTGACAAACAATGAAAAAGAATTTACTCGGATTAAAGAAATTAAAATTGAAAACTGGCTTT
AA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT192226 WP_000125566.1 NZ_CP069945:836702-836935 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT192226 NZ_CP092203:c3456964-3456734 [Leptospira weilii]
ATGAATAAAGCTAAAATTTTTAAAAATGGTGACAGCCAAGCAATACGTTTACCAAAGGACTATCGTTTTAAGGGTAAAGA
AGTTTATATACGAAAAGATGGCGATAACGTCATTTTAACGCCAATAGACGATGCGGTAGATCGATTTTGGAATACGGTTA
ATAATTTTTCTGAAGATCTGCTTATTGATCGGAATCAACCAAATGGCTACGATAAACGAAATAGAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References