Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 727211..727787 Replicon chromosome
Accession NZ_CP069945
Organism Escherichia coli strain FDAARGOS_1373

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag I6L03_RS03345 Protein ID WP_001350372.1
Coordinates 727211..727498 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag I6L03_RS03350 Protein ID WP_000063155.1
Coordinates 727485..727787 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6L03_RS03330 (722844) 722844..724175 - 1332 WP_000505211.1 restriction endonuclease subunit S -
I6L03_RS03335 (724165) 724165..725784 - 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
I6L03_RS03340 (725848) 725848..727014 - 1167 WP_000800824.1 restriction endonuclease -
I6L03_RS03345 (727211) 727211..727498 + 288 WP_001350372.1 BrnT family toxin Toxin
I6L03_RS03350 (727485) 727485..727787 + 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
I6L03_RS03355 (727821) 727821..728777 - 957 WP_001350371.1 GTPase -
I6L03_RS03360 (728788) 728788..728991 - 204 WP_000467859.1 YbdD/YjiX family protein -
I6L03_RS03365 (729109) 729109..731259 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T192223 WP_001350372.1 NZ_CP069945:727211-727498 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T192223 NZ_CP092201:25623-25964 [Leptospira weilii]
TTGGTAAAGAGTAGGAAATATACTCCTGAAAAAGGAGATATAGTTTGGCTGAACTTTACCCCGCAAGCAGGTCATGAACA
AAAAGGTCGTAGACCAGCTTTAGTTTTGTCACCTAAAGAATATAATTCGAAAACTGGATTAGCAATTTTCTGTCCGATTA
CTAGTAAGATTAAAGGTTATCCATTCGAGGTAACAATTAAATTGAAGAAAATTGATGGTGTAATTCTTTCTGATCAAGTG
AAAAACTTAGACTGGACTATTCGTGAAGCTGAATTTATAGAATCAATAAATAAAGTTTCTTTGAAAGAAGTTTTAGATAA
TATTAAGTTATTAGTATTTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT192223 WP_000063155.1 NZ_CP069945:727485-727787 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT192223 NZ_CP092201:25393-25629 [Leptospira weilii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGACGGAAGCCACGTTGAATTACAATATGAAGGGGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTAGAAGAAA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCAACTGGTAACTCAATAGGTAAAGAAGCTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References