Detailed information of TA system TA192222

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 442870..443367
• Replicon: chromosome
• Accession: NZ_CP069945
• Organism: Escherichia coli strain FDAARGOS_1373

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: I6L03_RS02020
• Protein ID: WP_001173343.1
• Coordinates: 443194..443367 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: I6L03_RS02015
• Protein ID: WP_072016751.1
• Coordinates: 442870..443166 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6L03_RS01995 (439599)	439599..440318	-	720	WP_000611288.1	two-component system response regulator DcuR	-
I6L03_RS02000 (440315)	440315..441946	-	1632	WP_001216466.1	sensor histidine kinase	-
I6L03_RS02005 (442127)	442127..442357	+	231	WP_001683478.1	4Fe-4S mono-cluster protein YjdI	-
I6L03_RS02010 (442369)	442369..442641	+	273	WP_000405647.1	GNAT family N-acetyltransferase	-
I6L03_RS02015 (442870)	442870..443166	+	297	WP_072016751.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
I6L03_RS02020 (443194)	443194..443367	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
I6L03_RS02025 (443486)	443486..445003	-	1518	WP_000003807.1	lysine--tRNA ligase	-
I6L03_RS02030 (445240)	445240..446697	-	1458	WP_000856826.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T192222 WP_001173343.1 NZ_CP069945:443194-443367 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T192222 NZ_CP092188:c142525-142199 [Deinococcus sp. KNUC1210]
TTGACCCTCGTTACTGCGCTCTACGACGCCGGTGTGCTCTACCCGTCCCTGATCAGGAACCTGCTGGTGCATCTCGCCAC
CTCCGGCCTGGTGGCGGCCCGCTGGACAGAGCAGATCCAAGAAGAATGGAGCCGCAACCTGTTGCAGGATCGCCCCGAAC
TGACCCCCGAGCGGCTGGCCCGAACCCGGCGGCTCATGGACGCGGCGGTTCCCGATGCCCTGGTCGAGGACGATCAGGCA
TTGATCGAACTTGAACGGTTTGAGCAGGTTGGGCTGATCCAAACCGCAGAAGCGCTCGCATCGCCGTCTAACTATCGAAT
CACCTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11501.05 Da
• Isoelectric Point: 4.1705

>AT192222 WP_072016751.1 NZ_CP069945:442870-443166 [Escherichia coli]
MEGKNKFNTYVVFFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT192222 NZ_CP092188:c142908-142522 [Deinococcus sp. KNUC1210]
ATGACTGCCCCCTTTATTCCTACCCCCGATGACACCCAAGCCGCCCAGCTCCAGCTCGACATGCTCCGTGCTCAGGCCGG
CCGCCTCCCCGTTCGACTCGCAGAAGTCCTGGACGGCCTCCTCCAGGAGCTCGCCGCCGGACGGGCCGTGCAGGTCATCA
CCCTCGAAGAGGAAATCAGCACCCAACAGGCCGCCGAGCTCCTGAACGTCAGCCGTCCGTATCTGGTGAAACTGGTGGAA
TCCGGCGCCCTCCCCCACCGCAAGGTCGGTCCCCGGCGGCGGCTCCACCTCCAAGACGTGCTGGCTTACCGGGCACGGCT
GGAAGCCCAGCGACAGGCTGCCCTGCAGGCGCTGGCCGATGACCTGCAAGAACTGGGCCTCGATTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41