Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53381..54117 Replicon plasmid unnamed1
Accession NZ_CP069944
Organism Klebsiella oxytoca strain FDAARGOS_1332

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag I6K62_RS27295 Protein ID WP_004098919.1
Coordinates 53381..53863 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag I6K62_RS27300 Protein ID WP_004098921.1
Coordinates 53851..54117 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K62_RS27270 48771..49076 + 306 WP_012540235.1 PTS sugar transporter subunit IIB -
I6K62_RS27275 49096..50430 + 1335 WP_049594012.1 PTS sugar transporter subunit IIC -
I6K62_RS27280 50469..51848 + 1380 WP_049594011.1 glycoside hydrolase family 1 protein -
I6K62_RS27285 51859..52170 + 312 WP_049594032.1 PTS lactose/cellobiose transporter subunit IIA -
I6K62_RS27290 52551..53165 + 615 Protein_41 Mig-14 family protein -
I6K62_RS27295 53381..53863 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
I6K62_RS27300 53851..54117 - 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
I6K62_RS27305 54293..54547 - 255 WP_004098924.1 hypothetical protein -
I6K62_RS27310 54623..54880 - 258 WP_004098928.1 hypothetical protein -
I6K62_RS27315 54929..55132 - 204 WP_004098931.1 hemolysin expression modulator Hha -
I6K62_RS27320 55470..55904 - 435 WP_004098933.1 hypothetical protein -
I6K62_RS27325 56120..57520 - 1401 WP_004098935.1 copper resistance membrane spanning protein PcoS -
I6K62_RS27330 57517..58197 - 681 WP_004098938.1 copper response regulator transcription factor PcoR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(G) / sul1 / qacE / ant(3'')-Ia - 1..216716 216716


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T192220 WP_004098919.1 NZ_CP069944:c53863-53381 [Klebsiella oxytoca]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T192220 NZ_CP092185:2740050-2740796 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACAAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT192220 WP_004098921.1 NZ_CP069944:c54117-53851 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192220 NZ_CP092185:2740797-2740967 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References